【作者】 蔣俊; 羅珍; 劉艷芳; 彭川叢; 吳迪; 周帥; 張勁松; 馮志勇; 楊焱;
【Author】 JIANG Jun1,2,3,LUO Zhen1,LIU Yanfang2,PENG Chuancong1,WU Di2,ZHOU Shuai2,ZHANG Jingsong2,FENG Zhiyong2,YANG Yan2 1Infinitus Company Ltd,Guangzhou,Guangdong 510000,China;2National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;3College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China
【機構(gòu)】 無限極中國有限公司; 農(nóng)業(yè)部南方食用菌資源利用重點實驗室國家食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點開放實驗室上海市農(nóng)業(yè)科學院食用菌研究所; 南京農(nóng)業(yè)大學生命科學學院;
【摘要】 以粗多糖得率和含量為評價指標,對水提醇沉制備猴頭菌多糖(Hericium erinaceuspolysaccharides,HEP)工藝中主要醇沉條件進行了篩選,得出猴頭菌多糖醇沉的最佳工藝條件:提取液的濃縮比(粗提液體積∶子實體干重,mL∶g)為3∶1,醇沉濃度70%,加入乙醇時濃縮液溫度為40℃,醇沉時間和靜置溫度分別為8 h和4℃。不同醇沉濃度得到的多糖分子量分布及體外免疫活性測定結(jié)果表明,醇沉濃度為40%時,得到的多糖大分子部分占的比例較高,活性較好。醇沉濃度越高,多糖含有的小分子物質(zhì)越多。
【Abstract】 The conditions used for ethanol precipitation of Hericium erinaceus polysaccharides from aqueous extracts of mushroom fruit bodies were optimized based on yield and polysaccharide content.Higher yields and polysaccharide contents were obtained under the following conditions: concentration ratio(extract volume∶dry weight of fruit body),3∶1(mL∶g);ethanol concentration used for precipitation,70%;initial temperature of concentrated extract,40 ℃;precipitation time and temperature,8 h and 4 ℃ respecti...
【關(guān)鍵詞】 醇沉工藝; 濃縮比; 醇沉濃度; 分子量分布; 免疫活性;
【Key words】 Ethanol precipitation; concentration ratio; ethanol concentration; immunological competence;
【基金】 上海市農(nóng)委重點攻關(guān)項目[編號:滬農(nóng)科攻字(2008)第8-2號]的部分研究內(nèi)容
【分類號】TQ461