【作者】 許峰; 劉宇; 趙爽; 王賀祥; 張國慶; 王守現(xiàn); 閆躍龍; 侯宇; 馮杉杉;
【Author】 XU Feng1,LIU Yu1,ZHAO Shuang1,WANG Hexiang2,ZHANG Guoqing3,WANG Shouxian1,YAN Yuelong 2,HOU Yu2,FENG Shanshan 21Institute of Plant and Environmental Protection,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,China;2State Key Laboratory for Agribiotechnology,China Agricultural University,Beijing 100193,China;3Key Laboratory of Urban Agriculture(North) of the Ministry of Agriculture,College of Biotechnology,Beijing University of Agriculture,Beijing 102206,China
【機(jī)構(gòu)】 北京市農(nóng)林科學(xué)院植物保護(hù)環(huán)境保護(hù)研究所; 中國農(nóng)業(yè)大學(xué)農(nóng)業(yè)生物技術(shù)國家重點實驗室; 北京農(nóng)學(xué)院生物技術(shù)學(xué)院農(nóng)業(yè)部都市農(nóng)業(yè)(北方)重點實驗室;
【摘要】 從秀珍菇超高壓保鮮的包裝袋中分離獲得WM1002菌株,以其基因組為模板擴(kuò)增獲得該菌株的16SrRNA基因的序列,結(jié)果表明:該菌株與芽孢桿菌屬菌株的基因序列同源性較高,GenBank登錄號為HQ730134;以Neighbor-Joining(N-J)方法構(gòu)建系統(tǒng)發(fā)育樹,結(jié)果表明菌株WM1002同已知菌Bacillus velezensisCR-502、B.vallis mortis DSM11031、B.methylotrophicus CBMB205和B.atrophaeusJCM9070的遺傳距離最近,相似性均達(dá)到99.8%,因此將菌株WM1002鑒定為Bacillussp.。
【Abstract】 A bacterial isolate,WM1002,obtained from packaged Pleurotus geesterani fruit bodies that had been processed using super high pressure technology,was identified as a strain of Bacillus based on 16S rRNA sequencing.The 16S rRNA sequence has been deposited in GenBank under the accession number,HQ730134.A phylogenetic tree consisting of 16S rRNA sequences from other Bacillus species and constructed using the neighbor-joining method revealed that isolate WM1002 exhibited a high genetic similarity(99....
【關(guān)鍵詞】 秀珍菇; 芽孢桿菌; 16S rRNA;
【Key words】 Pleurotus geesterani; Bacillus; 16S rRNA;
【基金】 北京農(nóng)學(xué)院基礎(chǔ)基金(編號:2017516002)的部分研究內(nèi)容
【分類號】S646