【作者】 林娓娓; 吳曉金; 郭倩; 趙明文; 王瑞娟; 周峰;
【Author】 LIN Weiwei1,2,WU Xiaojin2,GUO Qian2,ZHAO Mingwen 1,WANG Ruijuan2,ZHOU Feng2 1College of Life Science,Nanjing Agricultural University,Key Laboratory of Microbiological Engineeringof Agricultural Environment,Ministry of Agriculture,Nanjing,Jiangsu 210095,China;2Shanghai KeyLaboratory of Agricultural Genetics and Breeding,Institute of Edible Fungi,Shanghai Academy ofAgricultural Sciences,Shanghai 201106,China
【機(jī)構(gòu)】 南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院農(nóng)業(yè)部農(nóng)業(yè)環(huán)境微生物工程重點(diǎn)開放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室;
【摘要】 通過塵埃粒子計數(shù)器測定杏鮑菇工廠不同凈化區(qū)域中不同粒徑的塵埃粒子數(shù)量,并根據(jù)潔凈度等級國際標(biāo)準(zhǔn)和國家標(biāo)準(zhǔn)分析空氣潔凈度級別;采用平皿沉降法測定空氣中微生物數(shù)量。統(tǒng)計2009年工廠生產(chǎn)期39月,所有栽培種從接種至搔菌階段的污染瓶數(shù)按接種月份統(tǒng)計,并與該統(tǒng)計月份的微生物數(shù)量比較。結(jié)果表明,杏鮑菇工廠栽培區(qū)各凈化區(qū)域在檢測期間空氣潔凈度均較高,塵埃粒子數(shù)量、霉菌數(shù)量均與高度、溫度呈正相關(guān);細(xì)菌數(shù)量、霉菌數(shù)量與塵埃粒子數(shù)呈顯著正相關(guān)。高溫季節(jié),霉菌、細(xì)菌數(shù)量增加,此時生產(chǎn)污染率也達(dá)最高值,故認(rèn)為生產(chǎn)污染率與微生物群落變化相關(guān),高溫時,需加大降溫、除塵、消毒等措施的力度,方可降低微生物數(shù)量,減少污染率,該廠污染率在8‰以下,其潔凈度可作為生產(chǎn)參考值。
【Abstract】 The numbers of dust particles of different diameter and the microbial loads in seven designated clean areas within a Pleurotus eryngii commercial cultivation facility were determined and compared to the ISO 14644 standard of air cleanliness.We also compared the level of contamination occurring among the mushroom cultivation bottles and the microbial loads within the facility during the production period from March to September,2009.Our data indicated a relatively high level of cleanliness in the...
【關(guān)鍵詞】 凈化區(qū)域; 塵埃粒子; 霉菌; 細(xì)菌; 微生物;
【Key words】 Clean area; mould count; bacterial count; dust particle;
【基金】 上海市科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目(編號:09dz2200800);;國家科技支撐計劃重點(diǎn)項(xiàng)目(編號:2008BADA1B03)的部分研究內(nèi)容
【分類號】S646.141