【作者】 孫曉紅； 陳明杰； 汪虹； 馮愛萍； 潘迎捷；
【Author】 SUN Xiaohong1,2,3,CHEN Mingjie1,WANG Hong1,FENG Aiping1,PAN Yingjie3 1National Engineering Research Center of Edible Fungi;Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2College of Life Sciences,Nanjing AgriculturalUniversity,Nanjing,Jiangsu 210095,China;3Shanghai Ocean University,Shanghai 200090,China
【機(jī)構(gòu)】 國家食用菌工程技術(shù)研究中心農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所； 南京農(nóng)業(yè)大學(xué)生命技術(shù)學(xué)院； 上海海洋大學(xué)；
【摘要】 根據(jù)草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(sahh)全長序列設(shè)計(jì)兩個(gè)嵌套引物,與Takara LA PCR in vitro cloning試劑盒的接頭引物結(jié)合進(jìn)行巢式PCR擴(kuò)增,得到sahh上游調(diào)控序列,通過序列分析發(fā)現(xiàn),該序列含有1個(gè)TATA盒和4個(gè)CAAT盒。應(yīng)用MatInspector程序分析sahh上游調(diào)控序列,發(fā)現(xiàn)該區(qū)域存在1個(gè)耐鹽因子,1個(gè)熱激因子,2個(gè)DRE元件和2個(gè)ABA應(yīng)答元件。
【Abstract】 An upstream regulatory sequence of a S-adenosyl-L-homocysteine hydrolase gene(sahh) from Volvariella volvacea was amplified by nested PCR using two nested primers designed according to the full-length sequence of sahh and which were connected to the primer linker of the Takara LA PCR in vitro cloning box.The upstream sequence contained one TATA box and four CAAT boxes.MatInspector program analysis revealed one salt resistance factor,one heat shock factor,two drought-response binding factors(DRE)...
【關(guān)鍵詞】 S-腺苷-L-高半胱氨酸水解酶基因； 調(diào)控序列； 草菇；
【Key words】 Volvariella volvacea； S-adenosyl-L-homocysteine hydrolase gene； regulatory sequence；
【基金】 農(nóng)業(yè)部公益行業(yè)科研專項(xiàng)(編號(hào):nyhyzx07-008)的部分研究內(nèi)容
【分類號(hào)】S646.13