【作者】 潘鴻輝　謝意珍　李向敏　張智　余雄濤　焦春偉　蔡勉華　楊小兵

【Author】 PAN Hong-hui,XIE Yi-zhen,LI Xiang-min,ZHANG Zhi,YU Xiong-tao,JIAO Chun-wei,CAI Mian-hua,YANG Xiao-bing(Guangdong Institute of Microbiology,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Yuewei Edible Fungi Technology Co.Ltd.,Guangzhou Guangdong 510070)

【機(jī)構(gòu)】 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室廣東省微生物應(yīng)用新技術(shù)公共實(shí)驗(yàn)室;廣東粵微食用菌技術(shù)有限公司

【摘要】 應(yīng)用3種腫瘤細(xì)胞模型對(duì)未破壁靈芝孢子、機(jī)械破壁靈芝孢子和全靈芝剝壁孢子進(jìn)行抗腫瘤試驗(yàn)研究。結(jié)果表明,未破壁及不同破壁方式的靈芝孢子對(duì)人惡性乳腺癌細(xì)胞(MT-1)、人惡性淋巴癌細(xì)胞株(Jurkat)、人惡性腦腫瘤細(xì)胞(U343)均有不同程度的抑制作用,抑制效果強(qiáng)弱為全靈芝剝壁孢子>機(jī)械破壁靈芝孢子>未破壁靈芝孢子,表明破壁的確能提高靈芝孢子對(duì)腫瘤細(xì)胞的抑制作用,而且選擇破壁的方法也很重要,生物酶法破壁能使靈芝孢子的生物活性成分得到充分釋放,所以大大提高靈芝孢子的功效。

【Abstract】 The objective of this paper was to compare the inhibitory effects in intact Ganoderma spores with sporoderm-broken spores which were broken by enzymatic method and physical method on three kind of tumour cells including MT-1(Human Breast Cancer),Jurkat(Human T cell lymphoblast-like cell line) and U343(Human astrocytoma cell line).The experiments indicated that the inhibitory effects of Ganoderma spores on cancer cell growth were sporoderm-broken spores(broken by en-zymatic method) > sporoderm...

【關(guān)鍵詞】 靈芝孢子;破壁;腫瘤細(xì)胞

【Key words】 Ganoderma spores;Sporoderm-broken;Tumour cells

【基金】 廣東省科技型中小企業(yè)技術(shù)創(chuàng)新基金項(xiàng)目(ZX032280);;廣東省中國科學(xué)院全面戰(zhàn)略合作項(xiàng)目(2009B091300139)

【文獻(xiàn)出處】 中國食用菌, Edible Fungi of China, 編輯部郵箱 , 2010年05期

【DOI】CNKI:SUN:ZSYJ.0.2010-05-017