【作者】趙爽; 劉宇; 許峰; 王賀祥; 王守現(xiàn); 孟莉莉; 耿曉麗

【Author】ZHAO Shuang1; LIU Yu1; XU Feng1; WANG He-xiang2; WANG Shou-xian1; MENG Li-li1; GENG Xiao-li1(1.Institute of Plant and Environment Protection; Beijing Academy of Agriculture and Forestry Sciences; Beijing 100097; 2.State Key Laboratory for Agrobiotechnology and Department of Microbiology; China Agricultural University; Beijing 100193)

【作者機(jī)構(gòu)】北京農(nóng)林科學(xué)院植保環(huán)保研究所; 中國(guó)農(nóng)業(yè)大學(xué)農(nóng)業(yè)生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室

【摘要】 對(duì)本實(shí)驗(yàn)室保藏的4個(gè)姬松茸品種進(jìn)行了菌絲發(fā)酵,以酪蛋白、PNPP和ABTS為底物測(cè)定了菌絲體細(xì)胞內(nèi)的蛋白酶、磷酸酶和漆酶的活性。數(shù)據(jù)結(jié)果顯示,被測(cè)定的4個(gè)姬松茸品種均具有磷酸酶的活性,其中姬松茸(巴西)的活性最高為48.1 U·g-1;蛋白酶和漆酶活性的檢測(cè)中發(fā)現(xiàn),只有姬松茸7號(hào)和姬松茸11號(hào)具有活性,其中姬松茸7號(hào)具有較高的蛋白酶活性(95.2 U·g-)1,姬松茸11號(hào)具有較高的漆酶活性(58.6 U·g-)1。姬松茸7號(hào)和姬松茸11號(hào)同時(shí)具有3種酶的活性,可以作為深加工的優(yōu)勢(shì)材料。

【Abstract】 Four strains of Agaricus blazei were chosed,which were available in our lab.The activities of proteinase,phosphatase and laccase were studied.The results showed that all of the tested strains exhibited the phosphatase activity,and the phos-phatase activity of Agaricus blazei was the highest of 48.1 U·g-1.It was found that Agaricus blazei No.7 had the highest pro-teinase activity of 95.2 U·g-1 and Agaricus blazei No.11 had the highest laccase activity of 58.6 U·g-1.The other two strains were devoid of proteinase and laccase activities.In all,Agaricus blazei No.7 and Agaricus blazei No.11 can be considered as advanced strains in process engineering.

【關(guān)鍵詞】 姬松茸; 蛋白酶; 磷酸酶; 漆酶

【Key words】 Agaricus blazei; Proteinase; Phosphatase; Laccase

【基金】國(guó)家科技支撐計(jì)劃項(xiàng)目,農(nóng)牧業(yè)廢棄物資源菌業(yè)循環(huán)利用技術(shù)研究與示范(2007BAD89B07)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年03期

【DOI】CNKI:SUN:ZSYJ.0.2010-03-016
 
【分類號(hào)】S646.11