【作者】張微思; 羅孝坤; 張麗英; 張利菁; 朱萍

【Author】ZHANG Wei-si; LUO Xiao-kun; ZHANG Li-ying; ZHANG Li-jing; ZHU Ping(Kunming Edible Fungi Institute of All China Federation of Supply and Marketing Cooperative; Kunming Yunnan 650223)

【作者機(jī)構(gòu)】中華全國(guó)供銷合作總社昆明食用菌研究所

【摘要】 采用組織分離法,對(duì)采自云南中甸、麗江、武定、巍山4個(gè)地方的松茸進(jìn)行了分離培養(yǎng),并利用感官及ITS測(cè)序?qū)λ囵B(yǎng)的菌絲進(jìn)行了鑒定。結(jié)果表明,利用斜面培養(yǎng)基很好地誘導(dǎo)出了菌絲體母種,誘導(dǎo)率達(dá)75%;而將分離培養(yǎng)得到的菌絲體進(jìn)行液體培養(yǎng),菌絲生長(zhǎng)良好,且能進(jìn)行轉(zhuǎn)擴(kuò)和較大規(guī)模培養(yǎng);液體培養(yǎng)的菌絲體仍具有松茸的特殊氣味,證明可能為松茸的純培養(yǎng)物;經(jīng)ITS測(cè)序比較,采自中甸、麗江、武定、巍山的松茸子實(shí)體和其液體培養(yǎng)的菌絲體,其遺傳相似度分別為0.9970、1、0.9980、0.9980,表明取自不同地點(diǎn)的子實(shí)體與其相應(yīng)的培養(yǎng)物在種質(zhì)上是一致的。

【Abstract】 In this study,the mycelia of Tricholoma matsutake from Zhongdian,Lijiang,Wuding and Weishan were cultivated by the method of tissue separation and identified by the method of sense organ distinguishing and ITS sequencing.The results showed that the mycelia of maternal strain were well induced and the rate of induction is 75% on the slant medium of PDA+ yeast extract paste(0.5%).The mycelia gained above could grow very well and be cultivated in larger scale on liquid culture medium.The mycelia of liquid culture had special smell as which Tricholoma matsutake had,and this showed it were likely to be pure culture from Tricholoma matsutake.By ITS sequencing,the genetic identities,between mycelia of liquid culture and fruiting body from Zhongdian,Lijiang,Wuding and Weishan,were 0.997,1.00,0.998 and 0.998 respectively,that implied the mycelia were indeed from their corresponding fruiting body.

【關(guān)鍵詞】 松茸; 純培養(yǎng); 菌絲體; ITS鑒定

【Key words】 Tricholoma matsutake; Pure culture; Mycelium; ITS identification

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年03期

【DOI】CNKI:SUN:ZSYJ.0.2010-03-017

【分類號(hào)】S646.9