【作者】丁興紅; 溫成平; 丁志山; 范永升

【Author】DING Xinghong; WEN Chengping; DING Zhishan; FAN YongshengZhejiang Chinese Medical University; Hangzhou; Zhejiang 310053; China

【作者機(jī)構(gòu)】浙江中醫(yī)藥大學(xué)

【摘要】 采用低能離子射線誘變原生質(zhì)體選育桑黃菌株SH009,在確定誘變劑量效應(yīng)曲線的基礎(chǔ)上選擇了1.5×1015ions/cm2氮離子的誘變處理劑量。經(jīng)過對(duì)誘變原生質(zhì)體單個(gè)再生菌落的篩選,得到8株變異株,選取生長速度較快的5個(gè)菌株進(jìn)行搖瓶培養(yǎng),這5個(gè)菌株的菌絲體多糖和總黃酮產(chǎn)量均比出發(fā)菌株有所提高,其中P4的多糖和總黃酮產(chǎn)量分別比出發(fā)菌株提高了87.76%和73.24%。5個(gè)誘變菌株經(jīng)平板傳代5代以后的生長速度均保持穩(wěn)定,無明顯回復(fù)突變。結(jié)果表明,采用低能離子射線誘變桑黃菌株是一種有效的誘變育種方法。

【Abstract】 Low energy ion implantation technology was used to generate mutants of Phellinus igniarius SH009 with enhanced polysaccharide and flavonoid content.Protoplasts of P.igniarius were subjected to ion implantation and,after protoplast regeneration,eight mutants were selected on the basis of mycelial growth rate and growth vigor.Following four sub-cultures on potato dextrose medium,five mutants exhibiting stable mycelial growth rates were selected for polysaccharide and flavonoid analysis.Crude polysaccharide levels were significantly higher(p<0.05) in two mutants compared with the parent strain,and total flavonoid levels were significantly higher(p<0.05) in all five mutants.Highest increases in polysaccharide and flavonoid content were recorded with mutant P4,which were 87.76% and 73.24% higher,respectively compared with the parental levels.Our data demonstrate that low energy ion irradiation is a suitable method for breeding improved strains of P.igniarius.

【關(guān)鍵詞】 桑黃; 低能離子射線; 誘變; 原生質(zhì)體

【Key words】 Phellinus igniarius; ion implantation; mutation; protoplast

【基金】2008年浙江省高等學(xué)校優(yōu)秀青年教師資助計(jì)劃(編號(hào):71008809);; 2009年浙江省中醫(yī)藥青年課題研究計(jì)劃(編號(hào):2009YA003)的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期

【DOI】CNKI:SUN:SYJB.0.2010-02-003

【分類號(hào)】S567.39