【作者】陳自宏; 虞泓; 曾文波; 楊俊媛; 袁靜; 陳毅堅(jiān)

【Author】CHEN Zihong; YU Hong; ZENG Wenbo; YANG Junyuan; YUAN Jing; CHEN Yijian Yunnan Herbal Laboratory; Institute of Herb Biotic Resources; Yunnan University; Kunming; Yunnan 650091; China

【作者機(jī)構(gòu)】云南大學(xué)中草藥生物資源研究所云百草實(shí)驗(yàn)室

【摘要】 以蟲草素含量為指標(biāo),采用正交實(shí)驗(yàn)優(yōu)化了適合擬黑蟲草(Cordyceps nigrella)菌株cnig-56固體發(fā)酵的培養(yǎng)基,并探討了該菌株固體發(fā)酵的最佳條件。結(jié)果表明,菌齡為3d的擬黑蟲草菌種按10%接種量接入優(yōu)化的固體培養(yǎng)基上(配方為大米粉9.9%,青稞粉6.6%,玉米粉9.9%,蠶蛹粉3.3%,粗麩皮70.15%,KH2PO40.05%,MgSO40.1%)于24℃發(fā)酵培養(yǎng)30d,其產(chǎn)物的蟲草素含量最高為101μg/g。

【Abstract】 Orthogonal testing was used to optimize selected parameters affecting cordycepin production by Cordyceps nigrella (strain cnig-56) grown under solid-state conditions. Highest cordycepin levels (101 μg/g) were recorded after 30 d cultivation at 24 ℃ on a medium consisting of 9.9% rice powder,6.6% highland barley powder,9.9% corn powder,3.3% dried silkworm chrysalis powder,70.15% unrefined wheat bran,0.05% KH2PO4,0.1% MgSO4·7H2O,a solid:water ratio of 1:1.2,and using 3-day-old mycelium (10% w/w) as inoculum.

【關(guān)鍵詞】 擬黑蟲草; 固體培養(yǎng); 正交實(shí)驗(yàn); 蟲草素

【Key words】 Cordyceps nigrella; solid fermentation; orthogonal test; cordycepin

【基金】云南省自然科學(xué)基金重點(diǎn)項(xiàng)目(編號(hào):2008CC019)的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-019
 
【分類號(hào)】S567.35