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  • 蘑菇圈上四孢蘑菇個(gè)體間遺傳差異的ISSR分析


    【發(fā)布日期】:2010-05-17

    王波; 王一; 盧冰; 賈定洪
    四川省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 四川農(nóng)業(yè)大學(xué)資源環(huán)境學(xué)院

    【中文摘要】 對(duì)四孢蘑菇(Agaricus campestris L.:Fr.)形成的蘑菇圈形態(tài)和子實(shí)體發(fā)生進(jìn)行了調(diào)查,并對(duì)蘑菇圈上的四孢蘑菇個(gè)體間遺傳差異進(jìn)行了ISSR分析,建立遺傳關(guān)系聚類圖。結(jié)果表明,在生長(zhǎng)于四川西部平坦高原草地上,四孢蘑菇形成的蘑菇圈為圓環(huán)狀,蘑菇圈上生長(zhǎng)的植物明顯較蘑菇圈內(nèi)、外生長(zhǎng)的植物茂盛,同一蘑菇圈上生長(zhǎng)的子實(shí)體生長(zhǎng)期不完全一致;同一蘑菇圈上的四孢蘑菇個(gè)體之間相似系數(shù)為0.4730.755,不同蘑菇圈上的個(gè)體遺傳相似系數(shù)為0.3330.604;在相似系數(shù)0.524水平上,不同蘑菇圈上的四孢蘑菇個(gè)體分別聚為不同類群。

    【英文摘要】 The geometrical configurations of annular fairy rings formed by the growth of Agaricus campestris fruit bodies were investigated,and the genetic relationships between nine fruit bodies from within a single ring and one fruit body from each of other three rings were analyzed using ISSR markers.Grass growing at the ring circumference was more abundant compared with that growing within and outside the circumference,while fruit bodies at different stages of development were observed in the same fairy ring.Genetic similarities based on ISSR markers revealed similarity coefficients ranging between 0.473~0.755 and 0.333~0.604 for fruit bodies from the same fairy ring and from different fairy rings,respectively.A dendrogram constructed using the ISSR amplification data separated mycelia isolated from 12 fruit bodies growing in four different fairy rings into four groups at a similarity coefficient of 0.524.

    【中文關(guān)鍵詞】 四孢蘑菇; 蘑菇圈; 子實(shí)體; 遺傳差異; ISSR
    【英文關(guān)鍵詞】 Agaricus campestris; fairy ring; fruit body; genetic diversity; ISSR markers
    【基金】國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):30771503);; 農(nóng)業(yè)部948項(xiàng)目[編號(hào):2006-G11(A)-01];; 農(nóng)業(yè)部公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(編號(hào):nyhyzx07-008);; 四川省科技廳科技攻關(guān)項(xiàng)目(編號(hào):05NG001-016-2)的部分研究?jī)?nèi)容

    【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-000

     
     
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