方熹君; 許泓瑜; 陸震鳴; 許正宏
江南大學(xué)醫(yī)藥學(xué)院制藥工程研究室; 江南大學(xué)工業(yè)生物技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 本文以金黃色葡萄球菌(Staphylococcus aureus)為指示菌,研究了虎奶菇(Pleurotus tuber-regium)發(fā)酵產(chǎn)物不同組分的抑菌作用。結(jié)果表明,虎奶菇發(fā)酵產(chǎn)物對(duì)金黃色葡萄球菌有明顯的抑制作用;在1040 mg/mL的濃度范圍內(nèi),虎奶菇發(fā)酵液不同組分的抑菌作用大小依次為正丁醇萃取物>乙酸乙酯萃取物>萃余發(fā)酵液,正己烷萃取物無(wú)活性?；⒛坦骄z體乙醇提取物對(duì)金黃色葡萄球菌的抑制作用明顯。推測(cè)脂溶性小分子化合物為虎奶菇發(fā)酵產(chǎn)物抑菌的主要活性成分。

【英文摘要】 Pleurotus tuber-regium was grown in submerged culture,the mycelium removed by filtration,and the spent culture broth extracted sequentially with n-hexane,ethyl acetate and n-butanol.The latter two extracts and the residual broth remaining after extraction all exhibited antibacterial activity against Staphylococcus aureus over a range of concentrations,but no inhibition was recorded with the n-hexane extracts.Ethanolic and aqueous extracts of the fungal mycelium also showed potent antibacterial activity.

【中文關(guān)鍵詞】 虎奶菇; 液體發(fā)酵; 管碟法; 抑菌
【英文關(guān)鍵詞】 Pleurotus tuber-regium; submerged culture; well-plate method; antibacterial
【基金】國(guó)家“863”重點(diǎn)項(xiàng)目(編號(hào):2007AA021506);; 教育部新世紀(jì)優(yōu)秀人才支持計(jì)劃(編號(hào):NCET-07-0380)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-012