謝占玲; 何智媛; 唐龍清; 王學魯; 朱海梅; 楊家華; 灑威
甘肅農(nóng)業(yè)大學動物醫(yī)學院; 青海大學生物科學系

【中文摘要】 以菌絲體生物量和胞外多糖量為指標,分別采用不同碳源和氮源對5株羊肚菌[Morchella angusticeps、M.conica、Morchella sp.(50647)、Morchella sp.(50648)和M.esculenta]進行液體發(fā)酵,篩選出這5株羊肚菌的最佳碳源、氮源以及最適合液體發(fā)酵的菌株。實驗結(jié)果表明:5株羊肚菌最佳碳源均為可溶性淀粉,最佳氮源分別為:硫酸銨、蛋白胨、酵母膏、硝酸鈉、硝酸鈉。最適合液體發(fā)酵的菌株為Morchella angusticeps,其生長到第7天時菌絲體生物量最高,可達5.76g/L;第12天左右時胞外多糖產(chǎn)量最高,可達2.17g/L。

【英文摘要】 The effects of different carbon and nitrogen sources on mycelial biomass and extracellular polysaccharide production by five strains of Morchella grown in submerged culture are described.Soluble starch was the preferred carbon source for all five strains,whereas the preferred nitrogen sources for M.angusticeps,M.conica,Morchella sp.(strain 50647),Morchella sp.(strain 50648),and M.esculenta were ammonium sulfate,peptone,yeast extract,sodium nitrate and sodium nitrate,respectively.When all five strains were grown with their preferred carbon and nitrogen sources,highest mycelial biomass(5.76 g/L after 7 d)and extracellular polysaccharide(2.17 g/L after 12 d)yields were produced by M.angusticeps.

【中文關鍵詞】 羊肚菌; 碳源; 氮源; 菌絲體生物量; 胞外多糖
【英文關鍵詞】 Morchella spp; submerged culture; carbon source; nitrogen source; mycelial biomass; extracellular polysaccharide
【基金】青海省社會發(fā)展及基礎研究資助項目(編號:Z-610)的部分研究內(nèi)容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-010