陽飛; 張華山; 董殿波; 顧亞婧
湖北工業(yè)大學(xué)生物工程學(xué)院

【中文摘要】 本文對美味牛肝菌(Boletus edulis)液體發(fā)酵生產(chǎn)粗多糖的培養(yǎng)基和發(fā)酵條件進(jìn)行了篩選。在實驗范圍內(nèi),美味牛肝菌發(fā)酵生產(chǎn)粗多糖的最適培養(yǎng)基配方為:馬鈴薯10.0%,蔗糖2.0%,蛋白胨0.6%,KH2PO40.3%,MgSO4.7H2O0.2%,CaCO30.2%,ZnSO4.7H2O0.1%。在初始pH5.05.4,振蕩轉(zhuǎn)速120r/min,培養(yǎng)溫度2528℃,250mL三角瓶中裝液量40%,加10顆玻璃珠,發(fā)酵時間5d時搖瓶培養(yǎng)粗多糖產(chǎn)量最高,達(dá)3.161g/L。經(jīng)10L機(jī)械攪拌發(fā)酵罐放大動態(tài)實驗,研究pH值、溶氧、菌絲生物量(濕重)以及粗多糖產(chǎn)量的變化,可知最適發(fā)酵時間為96h。

【英文摘要】 The effects of selected culture parameters on polysaccharide production by a strain of Boletus edulis are described.Optimal conditions for small-scale polysaccharide production in submerged flask cultures were as follows:a fermentation medium consisting of 10% potato,2% sucrose,0.6% peptone,0.10% ZnSO4·7H2O,0.2% CaCO3,0.3% KH2PO4 and 0.2% MgSO4·7H2O,pH 5.0～5.4,120 r/min shaking speed,temperature 25～28 ℃,40% medium loading ratio,10 glass beads per flask,and a 5-day incubation period.Under these conditions,polysaccharide production reached a peak of 3.161 g/L.In 10-L fermenter cultures using the same medium,pH,temperature,a 50% medium loading ratio,a similar stirrer speed,and an aeration rate of 0.8 vvm,highest polysaccharide yields were obtained after four days.

【中文關(guān)鍵詞】 美味牛肝菌; 發(fā)酵條件; 搖瓶培養(yǎng); 發(fā)酵罐
【英文關(guān)鍵詞】 Boletus edulis; culture parameters; submerged flask culture; fermenter

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-018