忻雅; 阮松林; 王世恒; 馬華升; 王偉科; 王淑珍
杭州市農(nóng)業(yè)科學(xué)研究院
【中文摘要】 本研究利用RAPD標(biāo)記和EST-SSR標(biāo)記對10個(gè)不同來源的秀珍菇菌株進(jìn)行聚類分析。200條RAPD引物中127條有擴(kuò)增產(chǎn)物。引物可用率為63.5%。從NCBI數(shù)據(jù)庫中下載秀珍菇及相近側(cè)耳屬食用菌EST序列2167條,聚類比對后得到全長為713.541 kb的非冗余EST 1442條,搜尋后得到的29個(gè)EST-SSR全部設(shè)計(jì)引物,其中26對引物(89.7%)顯示多態(tài)性。根據(jù)17條RAPD核心引物和10對EST-SSR核心引物的擴(kuò)增結(jié)果,對10個(gè)秀珍菇菌株進(jìn)行了RAPD標(biāo)記、EST-SSR標(biāo)記及二者相結(jié)合的聚類分析。3種分析結(jié)果相近,且與菌絲、子實(shí)體生長特性分析結(jié)果相統(tǒng)一—10個(gè)供試菌株區(qū)分為5組:1～4號菌株為一組,6號和7號菌株為一組,8號和9號菌株為一組,5號和10號菌株各自為一組。
【英文摘要】 Amplification products were obtained from ten strains of Pleurotus geesteranus using 127 (63.5%) of 200 RAPD primers tested.A total of 2167 Expressed Sequence Tags (ESTs) from P.geesteranus and related Pleurotus species were downloaded from the NCBI database and,after clustering,1442 non- redundant ESTs with a total length of approximately 713,541 bp were obtained.Twenty-nine Expressed Sequence Tag- Single Sequence Repeats (EST-SSRs) were selected from these non-redundant ESTs and used to design EST-SSR primers.All 29 primer pairs were used for PCR amplification of genomic DNA extracted from the mycelia of ten P.geesteranus strains,and amplification products generated using 26 primer pairs (89.7%) exhibited polymorphisms.A dendrogram based on UPGMA (unweighted pair group method with arithmetic average) cluster analysis using combined data obtained using 17 RAPD core primers and 10 EST- SSR core primer pairs revealed that the 10 strains were clustered into five groups.
【中文關(guān)鍵詞】 秀珍菇; RAPD標(biāo)記; EST-SSR標(biāo)記; 聚類分析
【英文關(guān)鍵詞】 Pleurotus geesteranus; RAPD marker; EST-SSR marker; cluster analysis
【基金】杭州市珍稀食用菌科技創(chuàng)新服務(wù)平臺(編號：20052212Y10)的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年04期 【DOI】CNKI:SUN:SYJB.0.2008-04-008