馮翠萍; 程紅艷; 唐兵; 張杰; 常明昌
山西農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院; 山西農(nóng)業(yè)大學(xué)資源環(huán)境學(xué)院

【中文摘要】 采用超臨界CO_2萃取法提取靈芝孢子油,通過正交試驗(yàn)確定最佳萃取條件是:萃取壓力、溫度、時(shí)間分別為25 MPa、45℃、60 min,CO_2流量25 L/h,在此條件下出油率可達(dá)3.96%。靈芝孢子油具有清除NaNO_2和DPPH(1,1-Diphenyl-2-picrylhydrazyl)自由基的作用,并且其清除作用隨孢子油用量的增加而增加。

【英文摘要】 Orthogonal testing was used to optimize the conditions for extracting oil from Ganoderma lucidum spores using supercritical CO_2 fluid technology.Oil yields (3.96%) were highest under the following conditions:25 Mpa pressure,45℃extraction temperature,60 min extraction time and a CO2 flux of 25 L/h. Extracted spore oil was active in scavenging NaNO2 and DPPH (1,1-Diphenyl-2-picrylhydrazyl):NaNO_2 scavenging activity increased from 23.5% to 70.1% when the concentration of spore oil was increased from 1% to 5%,and DPPH scavenging activity increased from 20.2% to 40.5% when concentration of spore oil was increased from 1.25% to 10%.

【中文關(guān)鍵詞】 超臨界CO_2; 孢子油; 清除NaNO_2; DPPH自由基
【英文關(guān)鍵詞】 supercritical CO_2; spore oil; NaNO_2 scavenging; DPPH radical
【基金】山西省科技攻關(guān)項(xiàng)目“珍稀菇類茶樹菇、姬松茸以及靈芝的開發(fā)研究”(編號(hào)：021025)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年04期 【DOI】CNKI:SUN:SYJB.0.2008-04-018