桂明英; 郭永紅; 朱萍; 劉蓓; 趙博; 丁曉雯
昆明食用菌研究所; 西南大學(xué)食品學(xué)院

【中文摘要】 通過蠶豆根尖細(xì)胞微核實(shí)驗(yàn)和Ames實(shí)驗(yàn),研究了美味牛肝菌水提液的致突變作用,為其安全性評(píng)價(jià)提供基礎(chǔ)。實(shí)驗(yàn)結(jié)果表明,當(dāng)美味牛肝菌水提液的使用劑量從2.48 mg/mL增加到25.46 mg/mL時(shí),蠶豆根尖細(xì)胞的微核率(‰)由27.21±9.53增加到33.33±1.53,表明美味牛肝菌水提液對(duì)蠶豆根尖細(xì)胞產(chǎn)生一定的微核誘導(dǎo)作用;在相同劑量下,它們對(duì)10μg/mL環(huán)磷酰胺誘導(dǎo)產(chǎn)生的蠶豆根尖細(xì)胞微核抑制作用由完全抑制下降到很低的抑制水平。Ames實(shí)驗(yàn)證明,當(dāng)美味牛肝菌水提液的使用劑量由每皿3.5 mg增加到70 mg時(shí),TA97,TA98和TA102的回復(fù)突變率有一定幅度增加,但均未達(dá)到陰性對(duì)照的2倍。

【英文摘要】 The mutagenic potential of aqueous extracts of Boletus edulis fruit bodies was determined by measuring the rate of micronucleus formation in horse bean root tip(HBRT) cells and by the Ames test.The rate of micronucleus formation increased from control values of 21.10‰ ±2.02‰ to between 27.21‰ ±9.53‰ and 33.33‰ ±1.53‰ when HBRT cells were exposed to B.edulis extracts over the range 2.48～25.46 mg/mL.Lower concentrations(2.48～10.02 mg/mL) strongly inhibited the rate of micronucleus formation induced by 10 μg/mL cyclophosphamide whereas higher concentrations(25.46 mg/mL) had no significant inhibitory effect.The Ames test revealed that B.edulis extracts did not induce mutations involving frame shifts or base pair substitution,and the fungus is therefore relatively safe to consume.

【中文關(guān)鍵詞】 美味牛肝菌; 微核率; 蠶豆根尖微核實(shí)驗(yàn); Ames實(shí)驗(yàn)
【英文關(guān)鍵詞】 Boletus edulis; micronucleus formation; horse bean root tip cell; Ames test
【基金】云南省科研院所技術(shù)開發(fā)專項(xiàng)“云南野生食用菌新型產(chǎn)品加工技術(shù)工程化應(yīng)用推廣”(編號(hào):2005KFZX-05)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-011