高躍; 袁萍; 茅仁剛; 陳浙江; 田翠
上海師范大學(xué)生命與環(huán)境學(xué)院; 上海師范大學(xué)生命與環(huán)境學(xué)院; 上海師范大學(xué)生命與環(huán)境學(xué)院; 上海師范大學(xué)生命與環(huán)境學(xué)院; 上海師范大學(xué)生命與環(huán)境學(xué)院 上海200234; 上海200234; 上海200234; 上海200234; 上海200234

【中文摘要】 研究了不同酶種類、酶濃度、酶解溫度和時(shí)間、滲透壓穩(wěn)定劑、pH值及菌齡等因素對(duì)云芝原生質(zhì)體制備以及不同再生培養(yǎng)基對(duì)云芝原生質(zhì)體再生的影響。結(jié)果表明,菌齡3d的云芝菌絲體用3%溶壁酶,以pH5.5,0.4mol/L的KCl為穩(wěn)定劑,30℃酶解5h獲得的原生質(zhì)體產(chǎn)量最高;用RM1再生培養(yǎng)基培養(yǎng),原生質(zhì)體再生率最高。

【英文摘要】 We have examined the effects of selected parameters(nature and concentration of lytic enzyme,exposure time,temperature,nature,concentration and pH of osmotic stabilizer,mycelial age) on Coriolus versicolor protoplast production.Highest protoplast yields were obtained from 3-day-old mycelium after treatment with 3%(w/v) lywallzyme for 5 h at 30 ℃ using 0.4 mol/L KCl(pH 5.5) as osmotic stabilizer.Of six media tested,highest mycelium regeneration rates were obtained on RM1 medium consisting of 20%(w/v) potato,1%(w/v) yeast,0.3%(w/v) KH2PO4,0.15%(w/v) MgSO4,0.003%(w/v) Vitamin B1,0.4 mol/L KCl and 3%(w/v) agar.

【中文關(guān)鍵詞】 云芝; 原生質(zhì)體制備; 原生質(zhì)體再生
【英文關(guān)鍵詞】 Coriolus versicolor; protoplast preparation; protoplast regeneration

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:SYJB.0.2008-01-016