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    Identification of Tremella fuciformis strains Using SRAP, ISSR and RAPD markers


    【發(fā)布日期】:2010-05-17

    QU Shaoxuan; GAO San; HUANG Chenyang*Institute of Agricultural Resources and Regional Planning; Chinese Academy of Agricultural Sciences; Beijing 100081; China

    【中文摘要】 Three types of molecular markers (SRAP, ISSR and RAPD) were used to identify four Tremella fuciformis strains, T6 (white), T7 (white), T8 (yellow) and T9 (light yellow). Twelve SRAP primer pairs, ten ISSR primers and eight RAPD primers were screened, and identification data obtained using the three molecular markers were consistent in that the four T. fuciformis strains were divided into three groups with T7 and T9 clustered together in a single group. Each RAPD primer generated a higher average...更多 number of polymorphic bands than either the SRAP or ISSR primers, and the average similarity between the four strains was 81.34%. SRAP markers reflected more genetic information compared with the two other markers, and the average similarity was 68.98%. Genetic information reflected by ISSR markers was intermediate between SRAP and RAPD, and the average similarity was 77.48%.  還原

    【英文摘要】 Three types of molecular markers (SRAP, ISSR and RAPD) were used to identify four Tremella fuciformis strains, T6 (white), T7 (white), T8 (yellow) and T9 (light yellow). Twelve SRAP primer pairs, ten ISSR primers and eight RAPD primers were screened, and identification data obtained using the three molecular markers were consistent in that the four T. fuciformis strains were divided into three groups with T7 and T9 clustered together in a single group. Each RAPD primer generated a higher average number of polymorphic bands than either the SRAP or ISSR primers, and the average similarity between the four strains was 81.34%. SRAP markers reflected more genetic information compared with the two other markers, and the average similarity was 68.98%. Genetic information reflected by ISSR markers was intermediate between SRAP and RAPD, and the average similarity was 77.48%.

    【英文關鍵詞】 SRAP; ISSR; RAPD; Tremella fuciformis Berk.
    【基金】Sponsored by the Foundation for the Structural Opti mization of the Agricultural Industry (No .06-11-01B)

    【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年03期 【DOI】CNKI:SUN:SYJB.0.2007-03-002

     
     
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