袁麗銘; 忻茜; 李興玉
上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院; 上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院; 上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院 上海200234 ; 上海200234 ; 上海200234

【中文摘要】 應(yīng)用流式細(xì)胞分選儀(Flow cell sorter,FCS)和透射電子顯微鏡(Transmissional elec-tron microscope,TEM)等技術(shù)觀察了巴西蘑菇(Agaricus blazei)多糖誘導(dǎo)K562腫瘤細(xì)胞凋亡的試驗研究。試驗發(fā)現(xiàn),流式細(xì)胞儀可檢測到經(jīng)巴西蘑菇多糖誘導(dǎo)K562腫瘤96h后產(chǎn)生明顯的亞二倍體峰;電鏡下觀察到K562腫瘤細(xì)胞核固縮,核內(nèi)染色質(zhì)濃縮、凝聚,緊靠在細(xì)胞核膜邊沿,形成新月狀或環(huán)狀結(jié)構(gòu)等典型的凋亡細(xì)胞的形態(tài)變化及凋亡小體。結(jié)果表明,巴西蘑菇多糖具有誘導(dǎo)K562腫瘤細(xì)胞凋亡的作用。

【英文摘要】 The research on the apoptosis of K562 tumor cells induced by polysaccharide extracted from Agaricus blazei was carried out using advanced technology of flow cell sorter and transmissional electron microscope.The result showed that,visible apoptotic peak could be detected by flow cell sorter after being induced for 96h by polysaccharide extracted from Agaricus blazei.Furthermore,karyopyknosis,chromatin condensed and aggregated,hugging the edge of cell nucleus,showing crico-structure,crescent-structure and other typical morphologic changes of apoptotic cell as well as apoptotic body could be seen in K562 cells through electro-microscope.It was concluded that the apoptosis of K562 tumor cells could be induced by polysaccharide extracted from Agaricus blazei.

【中文關(guān)鍵詞】 巴西蘑菇多糖; 誘導(dǎo); K562腫瘤細(xì)胞; 細(xì)胞凋亡
【英文關(guān)鍵詞】 Polysaccharide extracted from Agaricus blazei; Induce; K562 tumor cell; Apoptosis
【基金】上海市高等院校科學(xué)技術(shù)發(fā)展基金項目“巴西蘑菇DNA反義核酸抗腫瘤的實驗研究”(編號:02DK09)的部分研究內(nèi)容

【文獻出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2005年03期 【DOI】CNKI:SUN:SYJB.0.2005-03-007