尚曉冬 ; 李明容 ; 王南 ; 譚琦
農(nóng)業(yè)部食用菌產(chǎn)品質(zhì)量監(jiān)督檢驗測試中心(上海) ; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室 ; 上海市農(nóng)業(yè)遺傳育種重點實驗室 ; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海201106

【中文摘要】 靈芝孢子粉稱樣量與孢子計數(shù)(血球計數(shù)板法)的回歸分析結(jié)果表明,兩者顯著相關(guān)。在此基礎(chǔ)上建立了靈芝破壁孢子粉破壁率的檢測方法。破壁率48.9%的標(biāo)準樣品,日內(nèi)相對標(biāo)準偏差(RSD,n=14)為10.34%,回收率(mean±SD,n=14)為94.9%±9.81%;破壁率68.8%、80.0%和90.6%的標(biāo)準樣品,日內(nèi)相對標(biāo)準偏差(RSD,n=10)分別為4.14%、3.43%和2.22%,回收率分別為101.2%±4.2%、98.76%±3.39%和99.73%±2.21%(mean±SD,n=10)。

【英文摘要】 <Abstrcat> Hemocytometer measurement based on the high degree of correlation between the spore mass and spore nos. has been developed for the determination of sporoderm-broken rate of Ganoderma lucidum. With the standard sample of 48.9% sporoderm-broken rate, the relative standard deviation of the recovery broken rate and the average recovery rate were proved to be 10.34% and 94.86%±9.81%(mean±SD, n=14)resp.. With the standard samples of 68.8%、80.0%and 90.6% sporoderm-broken rate, the relative standard deviations of the recovery broken rates and the average recovery rates were proved to be 4.14%, 3.43%, 2.22% and 101.2%±(4.2%, 98.76%)±3.39%, 99.73%±2.21%(mean±SD, n=10) resp..

【中文關(guān)鍵詞】 靈芝; 破壁孢子粉; 破壁率; 血球計數(shù)板
【英文關(guān)鍵詞】 Ganoderma lucidum; Sporoderm-broken spore powder; Sporoderm-broken rate; Hemocytometer

【文獻出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2005年02期 【DOI】CNKI:SUN:SYJB.0.2005-02-008