劉世強(qiáng); 楊麗卿; 郭麗瓊; 林俊芳
中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室; 中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室; 中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室; 中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室 ?？?71701 ; 海口571701 ; ?？?71701; 華南農(nóng)業(yè)大學(xué)食品學(xué)院生物工程系; 廣州510640 ; ?？?71701; 華南農(nóng)業(yè)大學(xué)食品學(xué)院生物工程系; 廣州510640
【中文摘要】 根據(jù)KajiwaraS[1]等報(bào)道的ras啟動(dòng)子的序列設(shè)計(jì)并合成一對(duì)特異引物,以草菇菌絲的總DNA為模板,通過(guò)PCR擴(kuò)增,獲得一條長(zhǎng)約0.75kb片段。DNA序列測(cè)定結(jié)果表明,該片段長(zhǎng)度為751bp。采用Blast軟件和Promoterpredictions軟件進(jìn)行啟動(dòng)子序列結(jié)構(gòu)分析,結(jié)果表明,該片段中243～293bp和539～589bp兩區(qū)域?yàn)閞as啟動(dòng)子的基礎(chǔ)啟動(dòng)子區(qū)。在283bp和579bp處有兩個(gè)轉(zhuǎn)錄起始位點(diǎn),在244～247bp和292～295bp之間有2個(gè)TATAbox,在36～39bp、377～380bp、434～437bp和716～719bp之間有4個(gè)CAATbox。此外,該片段中還含有9個(gè)Gbox、12個(gè)Ibox、2個(gè)Pbox以及3個(gè)重要順式作用元件:ABRE元件、WUN motif(基元)和HSE元件。
【英文摘要】 A ras promoter region about 0.75kb was cloned by PCR amplification with the primers designed according to the ras promoter sequence reported by Kajiwara S and template of genomic DNA from mycelium of Volvariella volvacea. DNA sequence determination results showed that the fragment length was 751bp. Sequence analysis with Blast software and Promoter predictions software indicated that the fragment contained two basic regions of ras promoter at the regions of 243～293bp and 539～589bp and two transcripted-originated sites at the regions of 283bp and 579bp, two TATA boxes at the regions of 244～247bp and 292～295bp, four CAAT boxes at the regions of 36～39bp,377～380bp,434～437bp and 716～719bp, and that it also contained nine G boxes, twelve I boxes, two P boxes, three important cis-acting elements: ABRE element, WUN-motif and HSE element which were different from the ras sequence from Lentinus edodes.
【中文關(guān)鍵詞】 草菇; ras啟動(dòng)子; PCR擴(kuò)增; 序列分析
【英文關(guān)鍵詞】 Volvariella volvacea; ras promoter; PCR amplification; Sequence analysis
【基金】國(guó)家自然科學(xué)基金資助項(xiàng)目(30060054和30371000);; 廣東省自然科學(xué)基金資助項(xiàng)目(032239);; 華南農(nóng)業(yè)大學(xué)校長(zhǎng)基金項(xiàng)目(5100-k03003)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年01期 【DOI】CNKI:SUN:SYJB.0.2004-01-001