肖建輝; 劉金偉; 劉祖林; 方寧; 肖瑜; 祁瑩; 萬衛(wèi)紅; 陳代雄; 梁宗琦
遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 遵義醫(yī)學(xué)院附屬醫(yī)院貴州省細(xì)胞工程實(shí)驗(yàn)室; 貴州大學(xué)真菌資源研究室 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州遵義563003 ; 貴州貴陽(yáng)550025

【中文摘要】 通過搖瓶液體培養(yǎng)實(shí)驗(yàn),確定江西蟲草培養(yǎng)基為蔗糖2%,甘油1%,黃豆浸液0.5%,酵母膏0.5%,KH2PO40.1%,MgSO40.05%,蒸餾水1000mL,初始pH6～7。裝液量200～250mL 500mL,加入10粒分散球,按培養(yǎng)液量的5%～7.5%接種6d左右菌齡的菌種,于26～28℃、旋轉(zhuǎn)式搖床150～180r min培養(yǎng)8d,最大菌絲體生物量可達(dá)16.6g L。

【英文摘要】 The culture conditions of Cordyceps jiangxiensis in shake flask culture were investigated. And the experiment results showed that the optimal compositions of submergod culture medium were as follows: sucrose 2%,glycerol 1%,soybean extract 0.5%,yeast extract 0.5%,KH_2PO_4 0.1%,MgSO_4 0.05%,distilled water 1000mL,initial pH 6～7. And the maximum mycelial biomass was as high as 16.6g/L when the medium capacity was 200mL～250mL in 500mL flask, 10 glass beads were used as dispersant, the seed of 6d age was inoculated at a rate of 5%～7.5%(V/V) and the flask culture experiment was carried out at 6～28℃ in a shake incubator with a rotation rate of 150r/min～180r/min for 8 days.

【中文關(guān)鍵詞】 江西蟲草; 菌絲體; 液體培養(yǎng); 生物量
【英文關(guān)鍵詞】 Cordyceps jiangxiensis; Mycelium; Submerged culture; Biomass
【基金】貴州省細(xì)胞工程重點(diǎn)實(shí)驗(yàn)室開放基金資助項(xiàng)目(No.02-003)

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年01期 【DOI】CNKI:SUN:SYJB.0.2004-01-006