陳美元; 廖劍華; 王澤生
福建省輕工業(yè)研究所; 福建省輕工業(yè)研究所; 福建省輕工業(yè)研究所 福州 350005 ; 福州 350005 ; 福州 350005

【中文摘要】 用72個隨機(jī)引物,對三種類型雙孢蘑菇菌株2796(雜交型)、8211(優(yōu)質(zhì)型)、01(高產(chǎn)型)等進(jìn)行RAPD擴(kuò)增,發(fā)現(xiàn)26個引物均產(chǎn)生三種不同的帶型。用該26個引物進(jìn)一步作三種類型9株雙孢蘑菇白色菌株的RAPD擴(kuò)增,發(fā)現(xiàn)引物U16、U20、B1、H4、H5、M7能很好地區(qū)分三類菌株,尤以U20和H5為佳,其產(chǎn)生的三種帶型穩(wěn)定整齊,差異帶明顯,為雙孢蘑菇菌株特性的早期預(yù)測提供了DNA水平的標(biāo)記。

【英文摘要】 Three types of A . bisporus strains including 27% ( hybrid type), 8211 (excellent - quality type) and 01 (high- yield type) were studied hy RAPD amplification reaction with 72 random primers. It was found that 26 primers all presented three different band patterns resp. . Further study was carried out with 26 primers and 9A. bisporus strains of three types. As a result, 6 primers--U16, U20, B1, H4, H5 and M7 were proved to heable to distinguish the three types of strains very well, especially primers U20 and H5, they were the best primers with stable and regular band patterns and obvious diversity bands, providing a DNA level marker for the prediction of the characteristics of A . bisporus strains.

【中文關(guān)鍵詞】 雙孢蘑菇; RAPD; 菌株類型
【英文關(guān)鍵詞】 Agaric us bisporus ; RAPD; Strain type
【基金】福建省自然科學(xué)基金資助

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1998年04期 【DOI】CNKI:SUN:SYJB.0.1998-04-001