詹才新; 張引芳; 凌霞芬; 賀冬梅; 嚴(yán)培蘭; 高紅亮
農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 河南農(nóng)業(yè)大學(xué) 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 鄭州 450002
【中文摘要】 本文以中國雙孢蘑菇不同主栽產(chǎn)區(qū)的9個具代表性的雙孢蘑菇菌株為材料,用20個隨機引物對其進(jìn)行RAPD分析。結(jié)果表明,20個隨機引物中,有15個隨機引物的擴增產(chǎn)物DNA片段表現(xiàn)出明顯的多態(tài)性,其中每個引物對不同菌株擴增出的DNA片段數(shù)目各不相同,而且不同引物擴增出的DNA帶譜也不同,差異較大。這15個引物對供試菌株總共擴增出168條DNA片段,其中最大的片段可達(dá)6.77kb,最小的DNA片段儀有0.31kb。同時,比較了供試菌株兩兩間的遺傳相似性,發(fā)現(xiàn)它們之間的變化不大,其相似系數(shù)從0.6891到1.0000,而平均相似系數(shù)僅為0.8500,表明供試菌株之間的親緣關(guān)系較近,遺傳變異不豐富。另外,采用系統(tǒng)聚類法中的類平均法,對供試菌株相似系數(shù)兩兩間進(jìn)行聚類分析并生成樹狀圖譜,直觀準(zhǔn)確地揭示了它們之間的差異。當(dāng)相似系數(shù)為77％時,所有供試菌株都被聚為一大類,說明我國主栽雙孢蘑菇菌株的遺傳基礎(chǔ)較狹窄,可能來源于同一品系,這為進(jìn)一步開展雙孢蘑菇菌種的遺傳改良提供了理論依據(jù)。
【英文摘要】 The random amplified polymorphic DNA(RAPD) analysis was conducted with 20 random primers in 9 representative strains of Agaricus bisporus collected from various major cultivation localities in China in this stady. The result revealed that 15 out of 20 random primers were scorable polymorphic ones. The number of scorable DNA fragments derived from each primer amplifying in various strains was different. Of each primer tested, a wide variation in band profiles was observed. In total, 168 band positions were scored (1/0) for all the tested strains. The size of the amplified DNA fragments ranged from 310 to 6770 base pairs. Meanwhile, the genetic similarity between eacli of all the tested strains differed slightly for the similarity coefficient ranged from 0.6891 to 1.0000. A total average coefficient of similarity was only 0.8500. This indicated that kinship was nearer and there was little genetic variation between strains tested. On the other hand, cluster analysis based on coefficient o...更多f genetic similarity between the strains tested was carried out by UPGMA. The dendrogram framed by cluster analysis could exactly show difference among them. All the strains tested were divided into one group when coefficient of similarity was 0.77. This conclusion implied that genetic foundation of major cultivation strains of Agaricus bisporus in China was narrow and their origin might be the same. These results provided us with the theoretical basis for deep going genetic improvement research of Agaricus bisporus strains in our country. 還原
【中文關(guān)鍵詞】 雙孢蘑菇; DNA多態(tài)性; RAPD分析; 聚類分析
【英文關(guān)鍵詞】 Agaricus bisporus; DNA polymorphism; RAPD analysis; Cluster analysis
【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1998年02期 【DOI】CNKI:SUN:SYJB.0.1998-02-000