蔣冬花; 張萍華
浙江師范大學(xué)生物系!金華321004; 浙江師范大學(xué)生物系!金華321004

【中文摘要】 本文研究了在500ml搖瓶振蕩培養(yǎng)條件下,培養(yǎng)基的初始pH值、不同碳源、不同氮源對金針菇菌絲體生長的影響和培養(yǎng)過程中培養(yǎng)液pH值的變化規(guī)律,并測定了菌絲體和子實(shí)體的主要營養(yǎng)成分及氨基酸含量。結(jié)果表明,深層培養(yǎng)7d,菌絲體生長量最高,培養(yǎng)液的pH值用培養(yǎng)時(shí)間的延長而逐漸下降,第7天最低;培養(yǎng)基最適pH為5.5～7.4;金針菇的適用碳源是葡萄糖、蔗糖和纖維素;適用氮源是蛋白胨和酵母膏。菌絲體與子實(shí)體的營養(yǎng)成分種類相似,而菌絲體的蛋白質(zhì)含量和氨基酸總量均高于子實(shí)體。

【英文摘要】 The effect of primary pH, different carbon sources, different nitrogen sources on mycelial growth and the pH changing law of liquid medium during the culture of Flammulina velutipes in 500ml shake flask were studied in this paper. The main nutrients and amino acid content in mycelia and fruitbodies were also analysed. The results showed that after submerged culture for seven days, the mycelial yield was the highest while the pH of the liquid medium decreased with the extending of culture time and fell to the lowest at the seventh day. The optimum pH of the medium was 5.5-7.4. The optimum carbon sources and nitrogen sources for F. velutipes were glucose, sucrose, cellulose, peptone and yeast extract resp.. The nutrient compositions of mycelia and fruitbodies were almost the same, but the content of protein and total amino acids in mycelia was higher than that in fruitbodies.

【中文關(guān)鍵詞】 金針菇; 深層培養(yǎng); 菌絲體
【英文關(guān)鍵詞】 Flammulina velutipes; Submerged culture; Mycelium

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1997年03期 【DOI】CNKI:SUN:SYJB.0.1997-03-006