潘迎捷; 陳明杰; 汪昭月; 譚琦; 馮志勇; 賀冬梅; 鄭連勝; 沈宗英
農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室!上海市農(nóng)業(yè)遺傳育種重點開放實驗室上海市農(nóng)業(yè)科學院食用菌研究所上海201106; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室!上海市農(nóng)業(yè)遺傳育種重點開放實驗室上海市農(nóng)業(yè)科學院食用菌研究所上海201106; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室!上海市農(nóng)
【中文摘要】 本文以香菇1號菌株的孢子單核體和原生質(zhì)單核體為材料,用統(tǒng)計學方法對兩類單核體的菌絲生長速度和羧甲基纖維素酶活性進行分析,并采用平均連鎖聚類方法對酯酶同工酶圖譜進行聚類,從交配型角度探討了不同來源的兩類單核體在形態(tài)和生化性狀上的差異。結果表明:孢子單核體和原生質(zhì)單核體在菌落形態(tài)、菌絲生長速度和羧甲基纖維素酶三個性狀上表現(xiàn)出相同的差異趨勢,在同一種交配型的個體間,孢子單核體的變異系數(shù)明顯大于原生質(zhì)單核體,在酯酶同工酶電泳圖譜這一性狀上,用聚類方法可以將絕大多數(shù)單核體歸入所屬的交配型范圍內(nèi)。這兩類單核體具有的不同表型與它們的不同來源有密切的聯(lián)系,有性世代中復雜的遺傳行為導致孢子單核體中性狀出現(xiàn)較大的變異,而作為無性后代的原生質(zhì)單核體則表現(xiàn)出性狀相對穩(wěn)定的特征,這兩類單核體表現(xiàn)出的差異為香菇的遺傳和育種研究提供了重要的基礎材料。
【英文摘要】 The mycelium growth rate, CMCase activity and esterase isoenzyme electrophoret-ogram of sporulated monokaryon and protoplasted monokaryon of Lentinula edodes strain No. 1 were analysed by statistics and UPGMA resp., while the differences in morphological and biochemical characteristics between two kinds of monokaryon were also investigated as viewed from the mating type. The results indicated that the difference tendency of sporulated monokaryon and protoplasted monokaryon in colony morphology, mycelium growth rate and CMCase activity was almost the same. The diversity coefficient of sporulated monokaryon with the same mating type was significantly greater than that of the protoplasted monokaryon. Most monokaryon could be classified to their mating types by UPGMA on the characteristic of the esterase isoenzyme electrophoretogram. Significant variation in the characteristics among sporulated monokaryon individuals were caused by complex genetic characters in generative progeny, while pr...更多otoplasted monokaryon, as vegetative progeny, showed relative genetic stability. The differences between protoplasted monokaryon and sporulated monokaryon provided important material for genetic and breeding study. 還原
【中文關鍵詞】 香菇; 孢子單核體; 原生質(zhì)單核體; 遺傳差異
【英文關鍵詞】 edodes; Sporulated monokaryon; Protoplasted monokaryon; Genetic diversity
【基金】上海市優(yōu)秀學科帶頭人計劃項目資助
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1997年02期 【DOI】CNKI:SUN:SYJB.0.1997-02-000