馬愛民 ; 賀冬梅 ; 凌霞芳 ; 潘迎捷
上海市農(nóng)業(yè)科學(xué)院食用菌研究所 ; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室 上海 201106

【中文摘要】 為建立雙孢蘑菇同核原生質(zhì)體雜交育種體系,本文對(duì)同核原生質(zhì)體親合性反應(yīng)及雜交異核體F_1代的農(nóng)藝性狀和F_2代的分離與變異規(guī)律進(jìn)行了研究。結(jié)果表明:雙孢蘑菇同核原生質(zhì)體的雜交是一個(gè)較復(fù)雜的性親合與核遷移過程,其雜交率較低。在按氣生型×氣生型、氣生型×匍匐型、匍匐型×匍匐型組合的520個(gè)雜交配對(duì)中,共獲得113個(gè)雜交異核體,平均雜交率為22%。本研究同時(shí)建立了以菌落形態(tài)、菌絲生長(zhǎng)速度、羧甲基纖維素酶相對(duì)活性以及酯酶同功酶電泳為手段的雜交異核體鑒定體系。

【英文摘要】 Compatible reaction of homokaryotic protoplasts, agronomic characters of heterokaryotic hybrid F1 generations, separation and variation of F2 generations were studied in this paper to establish a cross breeding system of homokaryotic protoplast in Agaricus bisporus. Results showed that cross between homokaryotic protoplast in Agaricus bisporus was a complicated procedure of sex compatibility and nuclear migration and its hybridization percentage was not high. 113 heterokaryotic hybrids were obtained from 520 combinations pairing with fluffy type x fluffy type, fluffy type x appressed type, appressed type x appressed type and average hybridization percentage was 22%. Meanwhile, identification system for heterokaryotic hybrid was also established by means of colonial morphology, mycelial growth rate, relative activity of Cx enzyme and esterase isozyme elecrophoresis.

【中文關(guān)鍵詞】 雙孢蘑菇; 同核原生質(zhì)體; 雜交育種; 親合性反應(yīng); 雜交異核體; F_1代與F_2代; 農(nóng)藝性狀; 分離與變異
【英文關(guān)鍵詞】 Agaricus bisporus; Homokaryotic protoplast; Cross breeding ; Compatible reaction; Heterokaryotic hybrid; F_1 and F_2 generation; Agronomic character; Separation and variation
【基金】上海市科委資助項(xiàng)目

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1995年04期 【DOI】CNKI:SUN:SYJB.0.1995-04-001