王謙; 李育岳; 楊曉先; 汪麟; 冀宏; 汪虹
河北省科學(xué)院微生物研究所!保定071051; 河北省科學(xué)院微生物研究所!保定071051; 河北省科學(xué)院微生物研究所!保定071051; 河北省科學(xué)院微生物研究所!保定071051; 河北省科學(xué)院微生物研究所!保定071051; 河北省科學(xué)院微生物研究所!保定071051

【中文摘要】 篩選菌株的發(fā)酵曲線表明,培養(yǎng)48～96小時為其增殖生長期;菌齡試驗表明89小時搖瓶菌種活力優(yōu)于113和137小時者;發(fā)酵罐中試培養(yǎng)中,64小時生物量即達(dá)33.7g/ml（鮮重）;醪液作為液體菌種,產(chǎn)量及菇形正常,發(fā)菌期縮短1/3且現(xiàn)蕾期較早,從醪液中可提取營養(yǎng)濃縮液及粗多糖粉。濃縮液含氨基酸555.38mg/100ml,多糖6.0mg/ml,可溶性固形物≥13％,急性毒性試驗證明無毒,重金屬含量符合國家標(biāo)準(zhǔn).

【英文摘要】 The fermentation curve of the selected strain of Flammulina velutipes indicates that the multiplication period is 48-96 hours. The activity of the shake flask spawn of 89 hours is better than that of 113 hours and 137 hours. The biomass is 33. 7g/100ml (fresh weight) at 04 hours in the fermenter. The spawn running time reduces one-third and primordiun formation is earlier than that of the normal cultivation when mash is used as liquid spawn. Concentrated nutrient liquid and coarse polysaccharide meal can be extracted from the mash. And the content of amino acid, polysaccharide and soluble solid is 555.38mg/100ml, 6.0mg/ml and >13% re?p. in the concentrated liquid. The concentrated liquid is proved safe by the acute toxic test and the content of heavy metal is in accord with the national standard.

【中文關(guān)鍵詞】 金針菇; 醪液; 液體菌種; 多糖
【英文關(guān)鍵詞】 Flammulina velutipes; Mash; Liquid spawn; Polysaccharide
【基金】河北省科學(xué)院重點課題

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1995年02期 【DOI】CNKI:SUN:SYJB.0.1995-02-012