康亞男; 練明忠; 鐘月金; 林懿寧; 陸勇軍; 康鈞玲
廣東省微生物研究所; 廣東省微生物研究所; 廣東省微生物研究所; 廣東省微生物研究所; 廣東省微生物研究所; 廣東省微生物研究所 廣州 510070 ; 廣州 510070 ; 廣州 510070 ; 廣州 510070 ; 廣州 510070 ; 廣州 510070
【中文摘要】 本研究采用種質(zhì)標(biāo)記法,以可出菇的交配基因型為遺傳標(biāo)記,以胞外酶種類,對底物專一性及酶活為生化標(biāo)記,通過對糖苷酶的篩選進(jìn)行育種。香雜26號是帶有種質(zhì)標(biāo)記的新菌株,遺傳標(biāo)記:A14B13×A1B2,生化標(biāo)記:內(nèi)源纖維素酶酶活:45mm及外源纖維二糖酶酶活:45mm。香雜26號與親本No.8.No.40酯酶同工酶譜比較,除同源酶帶外分別出現(xiàn)了互補(bǔ)型酶帶譜,顯示出強(qiáng)優(yōu)勢組合的新菌株。生物學(xué)特性表明,香雜26溫度適應(yīng)范圍廣(10～30℃),菌絲生長期短(60天),生物效率高(室內(nèi)栽培:118.44%比對照No.40增產(chǎn)62.8%,生產(chǎn)中試:90.98%),子實(shí)體中型勻稱,柄細(xì)而短,傘厚,含水量低。用交配基因型做遺傳標(biāo)記可將雜交組合控制在可出菇范圍。
【英文摘要】 The germplasm markers was adopted in the study with fruiting mating genotypes used as genetic markers and activities of various extracellular enzymes and their specificity for substrates as biochemical markers in the screening of gluco.sidase for L. edodes breeding. "Hybrid 26" was a new strain bearing A14B13×A1B2 as genetic markers, endo-cellulase activity 45 and exo-ceilobiase activity 45 ("Congo-red" assay) as biochemical markers. Esterase isozyme electrophoresis showed that "Hybrid 26" possessed esterase isozyme zymograms common to parental strains. In addition, there were also complementary bands among them, this indicated that "Hybrid 26" was a new strain from dominant cross combinations. "Hybrid 26" had a wide adaptation range of temperature (10-30℃), short mycelial growing cycle (60 days), high biological efficiency (118.44%) which was 62.8% higher than that of strain No.40. The biological efficiency was 90.98% in pilot production. It produced good quality mushrooms with medium...更多 and uniform size, short stipes, thick flesh and low moisture content. And genetic control of fruiting can be achieved in cross combinations using mating genotype as genetic markers. 還原
【中文關(guān)鍵詞】 香菇; 交配基因型; 遺傳標(biāo)記; 生化標(biāo)記; 育種
【英文關(guān)鍵詞】 Lentinus edodes; Mating genotype; Genetic markers; Biochemical markers; Breeding
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1994年02期 【DOI】CNKI:SUN:SYJB.0.1994-02-001