許曉燕; 鄭林用; 李艷; 江南; 余夢瑤; 羅霞
四川省中醫(yī)藥科學院中藥細胞與分子生物學實驗室; 四川省農(nóng)業(yè)科學院; 成都市經(jīng)濟信息中心

【中文摘要】 采用雙向固體發(fā)酵工程技術(shù),以靈芝為發(fā)酵菌株,在含有不同比例丹參邊角料的培養(yǎng)基中進行發(fā)酵,觀察了不同培養(yǎng)基中菌絲的生長情況,測定了發(fā)酵產(chǎn)物(靈丹菌質(zhì))中多糖、蛋白質(zhì)、三萜、丹參酮的含量變化,并研究了其提取物對小鼠活血化瘀功效的影響。結(jié)果表明靈丹菌質(zhì)的最佳發(fā)酵組合為組合4,發(fā)酵40d,多糖含量26.48μg·g-1,蛋白質(zhì)含量為397.24μg·g-1,靈芝酸三萜含量為4.92%。藥效學實驗顯示,靈丹菌質(zhì)組合4具有活血化瘀的藥效,優(yōu)于丹參藥材。

【英文摘要】 With two-way pattern solid-state fermentation technique, Ganoderma lucidum was fermented in the drug-containing medium containing different proportion of Salvia miltiorrhiza bunge by-product. Hyphal growth of G. lucidum was surveyed. The contents of polysaccharide, water-solubility protein, triterpene and tanshinton of solid-fermented products (SFP) were tested. In addition, the effect of activiting blood circulation to dissipate blood stasis on mouse fed with extracts was evaluated. The results showed that formulation 4 was the optimal medium, of which fermentation period was 40 d. Polysaccharide, crude protein, Ganoderma tanshinton of optimal SFP were 26.48 mg·g-1, 397.24 μg·g-1and 4.92%, respectively. From the results of pharmaco-dynamical experiments, the effects of SFP were better than that of Salvia miltiorrhiza.

【中文關(guān)鍵詞】 靈芝; 丹參邊角料; 雙向發(fā)酵; 藥效品質(zhì)
【英文關(guān)鍵詞】 Ganoderma lucidum; Salvia miltiorrhiza bunge by-product; Two-way pattern solid-state fermentation; Pharmacody- namic action
【基金】省中醫(yī)管理局項目;; 省應(yīng)用基礎(chǔ)項目(2008JY0006-2)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-013