馮偉林; 蔡為明; 金群力; 范麗軍
浙江省農(nóng)業(yè)科學(xué)院園藝所; 浙江大學(xué)農(nóng)業(yè)與生物技術(shù)學(xué)院

【中文摘要】 試驗(yàn)應(yīng)用ISSR技術(shù)分析了杏鮑菇生產(chǎn)性菌株的DNA序列多態(tài)性,從20個(gè)引物中篩選出11個(gè)ISSR引物對12個(gè)杏鮑菇生產(chǎn)性菌株基因組DNA進(jìn)行PCR擴(kuò)增。結(jié)果表明,這些杏鮑菇菌株遺傳相似系數(shù)為0.68～1.00。采用平均分類法UPGMA分析表明,在遺傳相似系數(shù)為0.83處可將這12個(gè)杏鮑菇生產(chǎn)性菌株分成3類。

【英文摘要】 Inter-simple sequence reqeats (ISSR) methods were used to analyze the DNA sequence polymorphism of cultivated Pleurotus erngii strains. 11 primers were chosed for ISSR among 20 primers. Genomic DNA of Pleurotus erngii were amplified by 11 primers using PCR. The results showed that the genetic similar coefficients (GSC) were 0.681.00. Cultivated strains were divided into 3 groups at GSC 0.83 based on the cluster analysis of UPGMA method.

【中文關(guān)鍵詞】 杏鮑菇; 遺傳差異; ISSR
【英文關(guān)鍵詞】 Pleurotus erngii; Genetic variation; ISSR

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-022