張普照; 舒任庚; 謝小梅
江西中醫(yī)學(xué)院現(xiàn)代中藥制劑教育部重點(diǎn)實(shí)驗(yàn)室; 江西中醫(yī)學(xué)院現(xiàn)代中藥制劑教育部重點(diǎn)實(shí)驗(yàn)室; 江西中醫(yī)學(xué)院菌物藥研究中心 江西南昌330004

【中文摘要】 目的:在前期藥理實(shí)驗(yàn)基礎(chǔ)上,分析雙向性固體發(fā)酵后雷公藤菌質(zhì)的化學(xué)成分。方法:采用高效液相色譜法和薄層色譜法,研究雷公藤藥材和不同發(fā)酵時(shí)間藥性菌質(zhì)的化學(xué)成分動(dòng)態(tài)變化規(guī)律。結(jié)果:藥性菌質(zhì)的雷公藤甲素含量低于原藥材低;分別在218nm和250nm波長(zhǎng)下,高溫滅菌藥材和發(fā)酵30d的藥性菌質(zhì)的液相圖譜有3處明顯的變化;乙酸乙酯提取物的薄層層析發(fā)現(xiàn)有一藍(lán)色斑點(diǎn)為發(fā)酵后新增成分,而甲醇提取物薄層層析發(fā)現(xiàn)有2個(gè)斑點(diǎn)隨發(fā)酵時(shí)間的延長(zhǎng)含量降低。結(jié)論:雷公藤經(jīng)藥用真菌發(fā)酵后化學(xué)成分發(fā)生變化,其中一些化學(xué)成分的變化規(guī)律與藥理實(shí)驗(yàn)結(jié)果存在相關(guān)性,可作為發(fā)酵終點(diǎn)判斷標(biāo)準(zhǔn)。

【英文摘要】 Objective:On the base of previous pharmacological and toxicological study,chemical compositions in Ttripterygium wilfordii HooK.F after fermentation was compared in this paper.Methods:The content of triptolide in each medicinal material was determined with HPLC and chemical constituents was analyzed with HPLC and TLC.Results:Triptolide content decreased after raw plant herbs was fermented with medicinal fungi,there were three characteristic absorption peaks at wavelength of 218nm and 250nm,one blue spot in TLC chromatograms of ethyl acetate extract and two spots gradually disappeared with the fermentation extent.Conclusion:The of chemical constituents in Tripterygium wilfordii HooK.F happened when medicinal material was fermented,there was relativity of chemical compositions and pharmacological toxicology,consequently,it was regarded as one standard of fermentation termination.

【中文關(guān)鍵詞】 雷公藤; 解毒持效; 雙相固體發(fā)酵; 藥用真菌
【英文關(guān)鍵詞】 Tripterygium wilfordii HooK.F; The deoxication and retentive-acting; Bi-directional solid fermentation; Medicinal fungal
【基金】國(guó)際科技合作項(xiàng)目(2006DFA33060)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:ZSYJ.0.2008-01-017