李祝; 肖洋; 梁宗琦
貴州大學(xué)生命科學(xué)院; 貴州省產(chǎn)品質(zhì)量監(jiān)測(cè)院; 貴州大學(xué)生命科學(xué)院 貴陽(yáng)550025; 貴陽(yáng)550025; 貴陽(yáng)550025

【中文摘要】 從疫霉Phytophthorasp.(YL)和曲霉As-pergillussp.(P6-1)兩株真菌菌絲體中分別提取其多糖作為激發(fā)子,在Cordycepsmillitaris97-0706菌株的不同生長(zhǎng)階段加入,觀察不同多糖激發(fā)子對(duì)97-0706菌株蟲草菌素含量的影響。結(jié)果表明:當(dāng)YL多糖單獨(dú)加入時(shí),僅對(duì)蟲草菌素含量有影響,而單獨(dú)加入P6-1多糖,也僅對(duì)生物量有影響。但在發(fā)酵0h,加入60μg/mLYL多糖,當(dāng)發(fā)酵到72h,再加入80μg/mLP6-1多糖,繼續(xù)發(fā)酵120h,蟲草菌素的含量可達(dá)到21.98±0.14mg/g(P<0.01),為對(duì)照的5.7倍,生物量可達(dá)到35.89±0.32g/l(P<0.01),比對(duì)照提高1.49倍。

【英文摘要】 The polysaccharide was extracted respectively from the mycelium of Phytophthora sp.(YL) or Aspergillus sp.(P6-1),and was used as elicitor at different mycelial growth phases of Cordyceps millitaris 97-0706 to observe the effect of the content for Cordycepin because there were different polysaccharide elicitors.The results showed that when only YL polysaccharide was added to it,it could only affect the content of Cordycepin,and when P6-1 polysaccharide was added to it,it only could affect the biomass.But when it fermented at 0h,60ug/ml YL polysaccharide was first added to it.Then 80ug/ml P6-1 polysaccharide was added to it after fermenting 72h.When it fermented up to 120h,the content of Cordycepin reached to 21.98±0.14mg/g(P<0.01),which was 5.7 times of CK,and biomass could reach to 35.89±0.32g/l(P<0.01),which was 1.49 times of CK.

【中文關(guān)鍵詞】 蟲草菌素; 真菌多糖; 激發(fā)子; 蛹蟲草; 生物量
【英文關(guān)鍵詞】 Cordycepin; Fungal Polysaccharide; Elicitor; Cordyceps millitaris

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2006年03期 【DOI】CNKI:SUN:ZSYJ.0.2006-03-012