王楠; 任大明; 龔濤; 關(guān)艷麗; 楊陽
遼寧沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院生物工程重點(diǎn)實(shí)驗(yàn)室; 遼寧沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院生物工程重點(diǎn)實(shí)驗(yàn)室; 遼寧沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院生物工程重點(diǎn)實(shí)驗(yàn)室; 遼寧沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院生物工程重點(diǎn)實(shí)驗(yàn)室; 遼寧沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院生物工程重點(diǎn)實(shí)驗(yàn)室 沈陽110161; 沈陽110161; 沈陽110161; 沈陽110161; 沈陽110161

【中文摘要】 采用60Co-γ射線輻照誘變尖端菌絲,在輻照劑量為800Gy和劑量率為27.84Gy/min的條件下選育出一株猴頭菌多糖高產(chǎn)菌株。其拮抗試驗(yàn)和過氧化物酶同工酶試驗(yàn)均證明其為變異株。經(jīng)液體培養(yǎng)其生物量和原始菌株相比提高14.2%,菌絲多糖產(chǎn)量提高2倍。經(jīng)6次傳代培養(yǎng)后,其生物量和菌絲多糖產(chǎn)量較穩(wěn)定,且明顯超過原始菌株,表明其遺傳穩(wěn)定性良好。

【英文摘要】 A high polysaccharide yield strain of Hericium erinaceus was screened through 60Co-gamma irradiation under the condition of 800 Gy of irradiation dose and 27.84 Gy/min of dose rate.It was identified by antagonism test and peroxidase isozyme isozyme electrophoresis.Compared with the original strain,the biology yield increased 14.22% and the polysaccharide yield increased about 2 times in liquid culture.After continuous cultivation for 6 generations,the biology yield and the polysaccharide yield were steady and obviously exceeded the original strain.It showed a good genetic stability.

【中文關(guān)鍵詞】 猴頭菌多糖; 60Co-γ射線; 尖端菌絲; 輻照劑量
【英文關(guān)鍵詞】 Hericium erinaceus polysaccharide; 60Co-gamma irradiation; Pointed hypha; Irradiation dose

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2005年06期 【DOI】CNKI:SUN:ZSYJ.0.2005-06-014