沙濤; 程立忠; 張漢波; 丁驊孫
云南大學(xué)工業(yè)微生物發(fā)酵工程重點(diǎn)實(shí)驗(yàn)室; 云南大學(xué)生物學(xué)系; 云南大學(xué)生物學(xué)系; 云南大學(xué)生物學(xué)系 昆明650091 ; 昆明650091 ; 昆明650091 ; 昆明650091

【中文摘要】 取松茸子實(shí)體的子實(shí)層制成粗松茸擔(dān)孢子懸液 ,采用脫脂棉過濾粗松茸擔(dān)孢子懸液 ,經(jīng) 40 0 0r/min3次離心沉淀微生物菌體、 80 μg/mL鏈霉素對(duì)細(xì)菌的殺菌作用 ,10 0 0 0r/min離心 ,收集得到純松茸擔(dān)孢子。此方法簡(jiǎn)單、雜菌污染率低、擔(dān)孢子收集效果明顯。

【英文摘要】 A simple method for collecting basidiospore of Tricholoma matsutake was introduced.Briefly,the gill of fruit body was flooded in the sterilized water containing 80μg/ml of streptomycin.Then,the fruit body debris was abandoned after the suspension was filtered by absorbent cotton and centrifuged at 4000r/min.The supernatant was centrifuged at 10000r/min again and the purified basidiospore were obtained.

【中文關(guān)鍵詞】 松茸; 擔(dān)孢子; 收集
【英文關(guān)鍵詞】 Tricholoma matsutake; Basidiospore; Collection
【基金】“十五”國家科技攻關(guān)計(jì)劃“生物資源與生物安全技術(shù)研究開發(fā)”項(xiàng)目 (No .2 0 0 1BA70 7B0 1)

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2003年03期 【DOI】CNKI:SUN:ZSYJ.0.2003-03-008