李林輝
四川師范學(xué)院生物系 四川南充637002

【中文摘要】 本研究以兩頭洞穿的玻璃管替代常規(guī)玻璃試管 ,制備兩端營(yíng)養(yǎng)較豐富而中間段營(yíng)養(yǎng)貧瘠但富含木質(zhì)素纖維素的夾心面包式固體培養(yǎng)基 ,利用大多數(shù)大型真菌分解利用木質(zhì)素纖維素能力較強(qiáng)的特點(diǎn) ,采用在一端接入野外不能嚴(yán)格消毒的子實(shí)體 ,卻在另一端取出已純化母種的方法 ,過濾掉雜菌 ,富集到目的大型真菌 ,為那些遠(yuǎn)離實(shí)驗(yàn)室地區(qū)考察短期內(nèi)不能將野外采集到的大型真菌立即送回實(shí)驗(yàn)室按現(xiàn)行普遍采用的方法立即進(jìn)行分離而獲得母種的大型真菌工作者提供了一個(gè)可資利用的新手段

【英文摘要】 This study provides a technology for the researchers who are working outdoors far from laboratory,who want to stay outdoors for longer,having no time to separate strains immediately from the macrofungi they dug by the popular method nowadays.The progress expresses as follows:preparing a glassy tube instead of popular one which is through,mixing similar-hamburger solid media which provides a abundant nutrition in both end where many animalcule may grow and spare one in the middle where only those animalcule which has the ability analyzing lignin and cellulose surely including macro fungi can grow,sterilization strictly,inoculating macrofungi which can not be sterilized strictly in one end of the media,culturing,a time latter,transferring pure hypha in another end of the media to PDA media or other media.

【中文關(guān)鍵詞】 大型真菌; 菌種; 技術(shù); 分離; 野外
【英文關(guān)鍵詞】 Macrofungi; Strain; Technology; Separate; Outdoors

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2002年05期 【DOI】CNKI:SUN:ZSYJ.0.2002-05-006