譚琦; 楊建明; 陳明杰; 賀冬梅; 潘迎捷; 黃為一
南京農(nóng)業(yè)大學(xué)資源與環(huán)境科學(xué)學(xué)院; 南京農(nóng)業(yè)大學(xué)資源與環(huán)境科學(xué)學(xué)院; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 南京農(nóng)業(yè)大學(xué)資源與環(huán)境科學(xué)學(xué)院 江蘇南京210095 ; 江蘇南京210095 ; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開放實(shí)驗(yàn)室; 上海市

【中文摘要】 本研究利用RAPD技術(shù) ,對香菇孢子單核體和原生質(zhì)體單核體的遺傳差異變化進(jìn)行了分析。 9個(gè)隨機(jī)引物共擴(kuò)增出 79條DNA帶 ,在此基礎(chǔ)上分析并構(gòu)建了遺傳相關(guān)聚類圖。結(jié)果表明以交配型基因?yàn)闃?biāo)記的孢子單核體遺傳變異大于原生質(zhì)體單核體 ,同種交配型的孢子單核體遺傳相似性分別為 6 6 3%和 71 7% ,而原生質(zhì)體單核體的遺傳相似性為 98 7%、 93 7%。

【英文摘要】 Sporulated monokaryon and protoplasted monokaryon of Lentinula edodes were studied for genetic diversity analysis by RAPD.A total of 79 products were amplified with 9 arbitrary primers and dendrogram based genetic similarity was performed from it.It was noted that more genetic diversity existed in sporulated monokaryon than that in protoplasted monokaryon at the same gene type.The similarity among same gene type of sporulated monokaryon were 66 3% and 71 7%.But the genetic similarity index of protoplasted monokaryon were 98 7% and 93 7%.

【中文關(guān)鍵詞】 香菇; 孢子單核體; 原生質(zhì)體單核體; RAPD; 遺傳分析
【英文關(guān)鍵詞】 Lentinula edodes; Sporulated monokaryon; Protoplasted monokaryon; RAPD; Genetic analysis

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2001年06期 【DOI】CNKI:SUN:ZSYJ.0.2001-06-000