胡文軍; 傅庭治; 曹幼琴
南京大學(xué)生物科學(xué)與技術(shù)系

【中文摘要】 本研究報導(dǎo)了液體深層培養(yǎng)金針菇（ＦｌａｍｍｕｌｉｎａｖｅｌｕｔｉｐｅｓＮＪ９６０１）的富硒能力和生物轉(zhuǎn)化。在添加５～１２５（×１０－６）硒的范圍內(nèi)，菌絲體富硒的范圍為８．２～９０．２（×１０－６）；添加２０（×１０－６）時，菌絲體吸收率最高，為對照的１８４％，此時菌體生長、可溶性蛋白質(zhì)、氨基酸、多糖等含量均高于對照組。無機硒經(jīng)菌體吸收、代謝、轉(zhuǎn)化，其有機化程度在７５％以上，分布在硒蛋白（占６４．１％），硒多糖（占１１．２％）。用反相高效液相色譜法定量分析硒蛋氨酸含量。用富硒金針菇粉和剩余培養(yǎng)液分別飼養(yǎng)蚯蚓，其產(chǎn)量可提高３０％以上。從飼養(yǎng)的蚯蚓提取分離獲得的蚓激酶產(chǎn)率提高２０％。開辟了富硒金針菇綜合利用的新途徑

【英文摘要】 In this paper, we report the ability of Flammulina uelutipes NJ9601, which cultivated in deep-liquid culture, to gather selenium and its biotransformation. When the selenium added to the culture is 5～125 (×10 -6 ), selenium contained in the hyphostroma is 8 2～90 2(×10 -6 ). When the selenium added to the culture is 20(×10 -6 ), the hyphostroma have the most selemium, which is 184% times more than that in the control. The dissoluble proteins, amino acids, polysaccharides in the hyphostroma wrer all more than those in the control groups. About 75% of nonorganic selenium that absorbed into the hyphostroma were transformed into organic selenium, which can be found in Se-proteins (64.1%), Se-polysaccharides(11.2%) and so on. The contents of Se-Met were quantitatively measured by reverse-high performance liquid chromotography. Applying the powders of Flammulina velutipes rich in selenium and the second-hand liquid cultures to earthworms, we found that the yield of earthworms could increase b...更多y 30% or more. And the yield of the fibrinolytic enzymes extracted from earthworms can increase by 20% or more. In this way, we open a new, comprehensive way to utilize Flammulina velutipes rich in selenium.  還原

【中文關(guān)鍵詞】 金針菇; 硒蛋氨酸; 富硒
【英文關(guān)鍵詞】 Flammulina velutipes; Se-Met; Se-concentration

【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1997年03期 【DOI】CNKI:SUN:ZSYJ.0.1997-03-019