陳穎; 朱繼紅; 雷秀云; 侯春平
遼寧大學(xué)生物系; 遼寧大學(xué)生物系; 遼寧大學(xué)生物系; 遼寧大學(xué)生物系 沈陽(yáng); 沈陽(yáng); 沈陽(yáng); 沈陽(yáng)
【中文摘要】 采用杯碟法和紙層析的方法,測(cè)定了榆耳發(fā)酵液對(duì)九種細(xì)菌的抑制作用。結(jié)果表明:榆耳發(fā)酵液對(duì)供試細(xì)菌均有不同程度的抑菌作用,尤其是對(duì)腸炎桿菌的抑制作用較為突出。用乙醇沉淀法除去多糖的榆耳發(fā)酵液,對(duì)腸炎桿菌、大腸桿菌、綠膿桿菌,金黃色葡萄球菌、炭疽桿菌仍表現(xiàn)出不同程度的抑制作用。用去多糖濃縮五倍的榆耳發(fā)酵液通過(guò)抗菌素常規(guī)的八個(gè)溶媒系紙層析顯影試驗(yàn),以腸炎桿菌為敏感菌展層,均未有明顯顯跡。
【英文摘要】 The inhibitory effects of the fermentation broth of Gloeostereum incarnatum have been determined by using the cup plate method and paper chromatography. The results showed that the broth had inhibitory effects in different degree to every strain of the nine bacteria tested, and there is an especially stronger effection to Salmonella enterilidis. The fermentation broth, whose polysaccharides have been eliminated by using the method of ethanol precipitation, still had inhibitory effects to S. enterilidis, E. coli, P. aeruginos and B. anthracis. The broth, when concentrated by five times, were tested in the conventional eight solvent systems paper chromatography, and S. enterilidis acted as sensitive strain, but the results showed little effects.
【中文關(guān)鍵詞】 榆耳; 發(fā)酵液; 紙層析; 杯碟法; 抑菌作用
【英文關(guān)鍵詞】 Gloeostereum incarnatum fermentation broth paper chromatography cup plate method
【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,1990年04期 【DOI】CNKI:SUN:ZSYJ.0.1990-04-001