高雄山蟲草人工栽培菌株篩選及搔菌對其子實(shí)體生物量的影響
Selection of an Anamorph of Cordyceps takaomontana for Artificial Cultivation,and Effect of ’Scratching’ on Fruit Body Biomass
【作者】 韓燕峰; 王寶林; 陳萬浩; 王玉榮; 鄒曉; 梁建東; 梁宗琦;
【Author】 HAN Yanfeng1,WANG Baolin1,CHEN Wanhao1,WANG Yurong1,ZOU Xiao1, LIANG Jiandong2,LIANG Zongqi1 1Institute of Fungal Resources,College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China; 2Department of Microbiology,School of Basic Medical Sciences,Guiyang College of Traditional Chinese Medicine,Guiyang,Guizhou 550002,China
【機(jī)構(gòu)】 貴州大學(xué)生命科學(xué)學(xué)院真菌資源研究所; 貴陽中醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院微生物教研室;
【摘要】 以小麥粒固體培養(yǎng)基栽培高雄山蟲草。篩選了適于生產(chǎn)的優(yōu)良菌株,該菌株栽培后生長良好,搔菌后出草較多、整齊,但生物量和生物轉(zhuǎn)化率與未搔菌相比沒有明顯差異;搔菌組第二潮子實(shí)體生物量和生物轉(zhuǎn)化率均優(yōu)于未搔菌組,搔菌組平均每瓶分別為(0.33±0.01)g和(2.2±0.01)%,未搔菌組分別為(0.12±0.01)g和(0.7±0.01)%。經(jīng)過二潮子實(shí)體培養(yǎng)總生物量和總生物轉(zhuǎn)化率高于一次培養(yǎng)。
【Abstract】 An anamorphic strain of Cordyceps takaomontana,GZUIFR-PT123,was selected for artificial cultivation based on the production of higher numbers of uniform(less branching) fruit bodies on potato dextrose agar.GZUIFR-PT123 mycelium was grown at 25 ℃ in culture bottles(13 cm high,6 cm diameter) containing a substrate mix consisting of 15 g wheat seeds,4 g silkworm chrysalis meal,0.5 g maltose,10 mL mineral salts solution(0.1% CaCl2,0.5% ZnSO4 and 0.5% MgSO4),5 mg vitamin B1and 25 mL water.After the g...
【關(guān)鍵詞】 高雄山蟲草; 搔菌; 子實(shí)體生物量; 生物轉(zhuǎn)化率;
【Key words】 Cordyceps takaomontana; scratching treatment; fruit body biomass; biological efficiency;
【基金】 國家自然科學(xué)基金(編號:30960004、31093440);貴州省教育廳自然科學(xué)基金(編號:2008059);貴州大學(xué)引進(jìn)人才科研項(xiàng)目(編號:2007-035)和貴州大學(xué)自然科學(xué)專項(xiàng)科研基金項(xiàng)目(編號:2010-102)的部分研究內(nèi)容
【分類號】S567.3