應(yīng)用iTRAQ結(jié)合2D LC-MS/MS技術(shù)分析草菇同核和異核菌絲蛋白質(zhì)組
Analysis of the Proteomes in Volvariella volvacea Homokaryotic and Heterokaryotic Mycelia by iTRAQ-coupled 2-D LC-MS/MS

【作者】 劉靖宇； 江玉姬； 謝寶貴； 陳炳智； 廖偉； 鄧優(yōu)錦； 劉朋虎；

【Author】 LIU Jingyu1,2,JIANG Yuji1,XIE Baogui1,CHEN Bingzhi1,LIAO Wei1,DENG Youjin1,LIU Penghu1(1Mycological Research Center of Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;2Institute of Food Science and Engineer,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

【機(jī)構(gòu)】 福建農(nóng)林大學(xué)菌物研究中心； 山西農(nóng)業(yè)大學(xué)食品科學(xué)與工程技術(shù)學(xué)院；

【摘要】 首次利用同位素標(biāo)記相對(duì)和絕對(duì)定量(isobaric tags for relative and absolute quantification,iTRAQ)技術(shù)結(jié)合二維液相色譜串聯(lián)質(zhì)譜對(duì)草菇(Volvariella volvacea)同核和異核菌絲蛋白質(zhì)組進(jìn)行研究。采用QExactive質(zhì)譜鑒定并經(jīng)MASCOT軟件搜庫(kù),對(duì)所有鑒定蛋白進(jìn)行主成分分析(principal component analysis,PCA)、層次聚類(hierarchical clustering)分析和GO(gene ontology)注釋,并進(jìn)行生物信息學(xué)分析。結(jié)果共獲得2335個(gè)不同肽段,鑒定到1039個(gè)蛋白,其中1030個(gè)蛋白具有定量信息。在同核菌絲中顯著上調(diào)蛋白83個(gè),下調(diào)蛋白97個(gè),表明iTRAQ標(biāo)記技術(shù)結(jié)合二維液相色譜串聯(lián)質(zhì)譜可有效地分離和鑒定草菇菌絲蛋白質(zhì)組。該研究結(jié)果為更深入全面地研究草菇菌絲蛋白質(zhì)組學(xué)奠定基礎(chǔ)。

【Abstract】 Protein expression in homokaryotic and heterokaryotic mycelia of Volvariella volvacea has been investigated using isobaric tags for relative and absolute quantification(iTRAQ)-coupled 2D LC-MS/MS(two-dimensional liquid chromatography tandem mass spectrometry).A total of 1039 proteins,including 2335 unique peptides,were identified using Q Exactive mass spectrometry and the MASCOT search engine,and 1030 of these had a quantitative dimension.Of the quantitatively different proteins,83 were signific...

【關(guān)鍵詞】 草菇； 蛋白質(zhì)組； iTRAQ； 二維液相色譜； 串聯(lián)質(zhì)譜；
 
【Key words】 Volvariella volvacea； proteome； isobaric tags for relative and absolute quantification(iTRAQ)； two-dimensional liquid chromatography tandem mass spectrometry(2D LC-MS/MS)；

【基金】 國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金資助(編號(hào):CARS 24);supported by the Engineering Research Center of EdibleFungi of Fujian Province,National ImprovementCenter of Edible Fungi Varieties(Fujian Subcenter)and the Institute of Biochemistry and Cell Biology,Shanghai Institute for Biological Science,Chinese Academy of Sciences~~

【分類號(hào)】S646.13