猴頭菌多糖罐批發(fā)酵種子培養(yǎng)及發(fā)酵條件優(yōu)化
Cultivation of Seeds and Optimization of Conditions for Batch Fermentation of Hericium erinaceus Polysaccharide
【作者】 張?bào)忝罚?朱維紅; 苗曉燕;
【Author】 ZHANG Xiao-mei,ZHU Wei-hong,MIAO Xiao-yan (Department of Biochemistry,Baoding University,Hebei Baoding 071000)
【機(jī)構(gòu)】 河北保定學(xué)院生化系;
【摘要】 試驗(yàn)確定了猴頭多糖罐批發(fā)酵的種子培養(yǎng)基配方、種子質(zhì)量標(biāo)準(zhǔn)、發(fā)酵控制條件及發(fā)酵終點(diǎn)。猴頭罐批培養(yǎng)要求種子活力旺盛,菌球色白,懸浮性強(qiáng),密度3.0×103個(gè).mL-1~4.0×103個(gè).mL-1,直徑0.5 mm~1.5 mm。5批發(fā)酵表明,猴頭多糖培養(yǎng)以26℃,pH5.0,攪拌150 r.min-1~200 r.min-1,罐壓0.04 PMa~0.06 PMa,通風(fēng)0.6 m3.h-1~0.8 m3.h-1培養(yǎng)144 h~168 h較適宜,菌絲多糖達(dá)115 mg.g-1~130 mg.g-1。比較發(fā)現(xiàn),猴頭罐批發(fā)酵較栽培子實(shí)體多糖高2倍。
【Abstract】 A recipe of substrate for Hericium polysaccharide seeds in fermenters,the quality index of seeds,the ferment conditions and termination conditions had found.Hericium in fermenters required thriving seeds,white fungus,high degree of suspension,with density of 3.0×103~4.0×103 per mL and diameter of 0.5 mm~1.5 mm.Five batches of ferment experiments had shown that when the cultivation of Hericium polysaccharide under 26℃,pH5.0,stirred at 150 r·min-1~200 r·min-1,with pressure of 0.04 MPa~0.06 MPa and...
【關(guān)鍵詞】 猴頭菌; 種子指標(biāo); 發(fā)酵罐; 多糖; 培養(yǎng)條件;
【Key words】 Hericium erinaceus; Seed index; Fermenter; Polysaccharide; Condition;
【基金】 河北省教育廳計(jì)劃指導(dǎo)研究項(xiàng)目(Z2008401)
【分類號(hào)】S646.9