不同產(chǎn)地猴頭菌子實(shí)體粗多糖特征及其刺激巨噬細(xì)胞產(chǎn)生NO的活性
Partial Characterization of Polysaccharides from Seven Hericium erinaceus Fruit Body Samples and Their Effect on Nitric Oxide Release from Macrophages
【作者】 吳迪; 張勁松; 蔣俊; 周帥; 汪雯翰; 劉艷芳; 馮娜; 唐慶九; 羅珍; 楊焱;
【Author】 WU Di1,ZHANG Jingsong1,JIANG Jun1,2,ZHOU Shuai1,WANG Wenhan1, LIU Yanfang1,FENG Na1,TANG Qingjiu1,LUO Zhen3,YANG Yan1 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China; 2College of Life Sciences,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China; 3Infinitus(China) Company Ltd,Guangzhou,Guangdong 510000,China
【機(jī)構(gòu)】 農(nóng)業(yè)部南方食用菌資源利用重點(diǎn)實(shí)驗(yàn)室國(guó)家食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院; 無(wú)限極(中國(guó))有限公司;
【摘要】 提取7種不同來(lái)源的猴頭菌(Hericium erinaceus)子實(shí)體粗多糖,對(duì)其理化性質(zhì)和體外免疫活性進(jìn)行分析。結(jié)果表明:不同產(chǎn)地的猴頭菌子實(shí)體粗多糖含量和得率差異較大,以來(lái)源于黑龍江省牡丹江市海林縣(H1)、林口縣(H3)的子實(shí)體粗多糖含量、得率較高;各粗多糖提取物分子量分布相似,單糖組成均以葡萄糖為主,并含有巖藻糖和半乳糖;7種子實(shí)體粗多糖提取物均能增強(qiáng)小鼠巨噬細(xì)胞株RAW264.7生成NO的量,以500μg/mL H3提取物活性最高、H1次之。
【Abstract】 Crude polysaccharide preparations were prepared by hot(100 ℃) water extraction and ethanol precipitation from Hericium erinaceus fruit bodies artificially cultivated at seven different facilities located in Fujian,Zhejiang and Heilongjiang Provinces of China.Highest yields of crude extract containing the highest amounts of polysaccharide were obtained from fruit body samples(H1 and H3) grown in Heilongjiang.HPLC analysis revealed that crude polysaccharide material extracted from the seven fruit ...
【關(guān)鍵詞】 猴頭菌; 粗多糖; 一氧化氮釋放; 巨噬細(xì)胞; 免疫調(diào)節(jié);
【Key words】 Hericium erinaceus; polysaccharide; nitric oxide production; macrophages; immunoregulation;
【基金】 上海市農(nóng)委重點(diǎn)攻關(guān)項(xiàng)目[編號(hào):滬農(nóng)科攻字(2008)第8-2號(hào)]的部分研究?jī)?nèi)容
【分類號(hào)】S646.9