【作者】 楊玉紅； 劉芳； 康宗利； 段玉璽；

【Author】 YANG Yu-hong1,2,LIU Fang2,KANG Zong-li2,DUAN Yu-xi1(1.Plant protection Post-doctoral Flow Station in Shenyang Agriculture University,Shenyang Liaoning 100866; 2.Biological Science Technology Institute of Shenyang Agriculture University,Shenyang Liaoning 100866)

【機(jī)構(gòu)】 沈陽農(nóng)業(yè)大學(xué)植物保護(hù)博士后流動(dòng)站； 沈陽農(nóng)業(yè)大學(xué)生物科學(xué)技術(shù)學(xué)院；

【摘要】 隨著生產(chǎn)規(guī)模的不斷擴(kuò)大,北蟲草的培育也暴露了一個(gè)問題,正常菌種經(jīng)多次轉(zhuǎn)管繼代和冰箱久存之后,會(huì)出現(xiàn)不產(chǎn)子實(shí)體現(xiàn)象,這些問題嚴(yán)重干擾了蟲草的生產(chǎn)。為此,進(jìn)行了有關(guān)北蟲草不同部位組織分離和繼代培養(yǎng)的試驗(yàn),并篩選出性狀優(yōu)良的菌株。結(jié)果表明,子實(shí)體靠近頂端分離的菌種易于萌發(fā),孢子分離得到的菌株性狀更好,北蟲草子實(shí)體本身就具有抑菌作用,因此,在選取表面消毒方式時(shí),一定要適宜,不可時(shí)間過長,以免對子實(shí)體造成傷害,不產(chǎn)生菌絲。

【Abstract】 With the procreative scale dilating constantly,the culture of Cordyceps sinensis had some questions.when the normal bacterium was transferred many times or be kept in the fridge for a long time,it maybe didn’t produce the fructification.So an experiment on the Cordyceps military tissue isolation and continuous culture were done,and a strong spices were got.The results showed that the part near top was more likely to sprout.North Cordyceps was some kind of bacteriostat.The one which isolated from...

【關(guān)鍵詞】 北冬蟲夏草； 人工栽培； 組織分離； 子實(shí)體；

【Key words】 Cordyceps sinensis； Artificial cultivation； Tissue isolation； Fructification；

【分類號(hào)】S646.9