【作者】 趙源; 劉艷芳; 蔣??; 楊焱; 唐慶九; 周帥; 吳迪; 賈薇; 馮娜; 張勁松;
【Author】 ZHAO Yuan1,2,LIU Yanfang1,JIANG Jun1,2,YANG Yan1,TANG Qingjiu1, ZHOU Shuai1,WU Di1,JIA Wei1,FENG Na1,ZHANG Jingsong1 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China; 2College of Life Sciences,Nanjing Agriculture University,Nanjing,Jiangsu 210095,China
【機(jī)構(gòu)】 農(nóng)業(yè)部南方食用菌資源利用重點(diǎn)實(shí)驗(yàn)室國家食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【摘要】 用水提醇沉法從6個(gè)不同產(chǎn)地毛頭鬼傘(Coprinus comatus)子實(shí)體中獲得6種粗多糖,6種粗多糖得率及多糖含量差異較大,以湖北(HB)的最高,其次為云南(YN)的;各粗多糖分子量分布相似,單糖組成均以葡萄糖為主,并含有少量半乳糖;除福建(FJ)外,其余5種粗多糖均能增強(qiáng)小鼠巨噬細(xì)胞RAW264.7釋放NO的產(chǎn)量,以YN和HN的活性較好,總體來說,YN的多糖產(chǎn)量及活性較好。
【Abstract】 Crude polysaccharide preparations were obtained from artificially-cultivated Coprinus comatus fruit bodies,provided by six commercial growers from different locations within China,by hot(100 ℃) water extraction and ethanol precipitation.Percentage yields of crude extracted material from the six samples were HB 9.42,YN 8.89,AH 8.52,HN 7.03,FJ 6.68 and ZJ 5.26,and the corresponding percentage yields of polysaccharide were 7.49,6.68,6.36,5.33,4.35 and 2.84.HPLC analysis revealed that crude polysacc...
【關(guān)鍵詞】 毛頭鬼傘; 多糖; NO產(chǎn)量; 巨噬細(xì)胞; 免疫調(diào)節(jié);
【Key words】 Coprinus comatus; polysaccharide; nitric oxide production; macrophages; immunoregulation;
【基金】 上海市科委成果轉(zhuǎn)化項(xiàng)目(編號(hào):113919N1300)的部分研究?jī)?nèi)容
【分類號(hào)】S567.39