Analgesic Substance from The Anamorph of Cordyceps gunnii(Berk.)Berk.

Abstract：A sort of analgesic substance is extracted from the mycelia of Paecilomyces gunnii Liang,an anamorph of Cordyceps. Some physical and chemical characters of this substance are elucidated. The analgesic substance from the mycelia of Paecilomyces gunnii Liang & Liu is water soluble. The best extractive method from the mycelia is soaking-heating extraction. The analgesic substance can be solved in 90% alcohol ,but can't be solved in ether or methanol.The molecular weight of the analgestic substance from Paecilomyces gunnii Liang & Liu is 12 000～14 000 Da. Infrared spectrum of the analgesic substance shows that it doesn't contain the structure of Dolantin's group.The analgesic substance is stable in pH <10 and it is also stable under 100 oC for one hour.In the dosage range of 0.03 g/g (raw herb/mice), the inhibition rate of writhing in mice induced by acetic acid is positively correlated to the dosage injected into mice. The analgesic substance of Paecilomyces gunnii Liang & Liu exists not only endocellular but also extracellular.

Keywords：Cordyceps gunnii; Paecilomyces gunnii; analgesic substance

古尼蟲草Cordyceps gunnii(Berk.)Berk.無(wú)性型的鎮(zhèn)痛物質(zhì)研究

梁宗琦　 常勝軍　 劉愛(ài)英　

摘　要：報(bào)道了分離提純自古尼蟲草(Cordyceps gunii)一個(gè)單子囊孢子的無(wú)性古尼擬青霉(Paecilomyces gunii)5-19菌株,菌絲培養(yǎng)物鎮(zhèn)痛組分的基本理化性質(zhì)和菌絲不同培養(yǎng)條件對(duì)其藥理作用的影響.結(jié)果表明:蟲草菌絲的鎮(zhèn)痛組分是水溶性的;鎮(zhèn)痛組分最佳提取溶劑為水,最佳提取方法為熱提取法;古尼蟲草鎮(zhèn)痛組分對(duì)冰醋酸所致小鼠扭體抑制率在劑量0.05 g/g(生藥/小鼠)范圍與劑量成正相關(guān);在所試pH值范圍內(nèi)(pH1～10),其鎮(zhèn)痛效果無(wú)明顯變化.當(dāng)pH>11時(shí),鎮(zhèn)痛作用強(qiáng)度下降.鎮(zhèn)痛組分有較好的熱穩(wěn)定性.在100 ℃加熱1 h,它對(duì)冰醋酸所致的小鼠扭體抑制率仍為100%;培養(yǎng)基組成和培養(yǎng)溫度在所試條件下,不僅影響菌絲的生物量,而且影響菌絲浸出物的鎮(zhèn)痛效果.

關(guān)鍵詞：古尼蟲草; 古尼擬青霉; 鎮(zhèn)痛物質(zhì)

CLC Number：S567.9

Author Resume：Liang Zongqi,male,born in 1936, professor. Address: Institute of Fungus Resources, Guizhou University, Guiyang 550025

Author Unit：梁宗琦（貴州大學(xué)真菌資源研究所,貴陽(yáng),550025）　

　　　　　常勝軍（貴州大學(xué)真菌資源研究所,貴陽(yáng),550025）　

　　　　　劉愛(ài)英（貴州大學(xué)真菌資源研究所,貴陽(yáng),550025）　

References：

［1］Liang Z Q. Biodiversity of the genus Cordyceps and its anamor-phs. Fung Sci, 1997,12(1,2):51～57

［2］Pointing S B, Hyde K D. Bio-exploitation of Filamentous fungi. Hong Kong :The University of Hong Kong Press, 2001. 1～451

［3］Liang Z Q. Isolation and identification of the conidial stage of Cordyceps gunnii. Acta Mycologica Sinica,1983, 4(3):162～166

［4］Liang Z Q, Liu A Y, Dong X C, et al. Application evaluation of Cordyceps gunnii. In Study and Application of Entomogenous Fungi in China,Vol. II.In: Division of Entomogenous Fungi,Chinese Society of Mycology eds. Study and Application of Entomogenous Fungi in China. Beijing: China Agricultural Scientech Press, 1990. 74～80

Manuscript Received：2003年11月3日

Published：2004年2月1日