突臍孢屬Brnl基因核苷酸序列比較及系統(tǒng)發(fā)育研究

SEQUENCE COMPARISON AND PHYLOGENETIC ANALYSIS OF BRN1 GENES FROM EXSEROHIL UM SPECIES

孫廣宇　張雅梅　張榮　

摘　要：對所有供試突臍孢菌株的Brnl基因(1,3,8-三羥基萘還原酶基因)擴增均獲得PCR產(chǎn)物.序列比較表明:在種內(nèi)各菌株間沒有核苷酸序列長度變化,存在核苷酸序列簡單代換;在種間核苷酸序列長度有變化,核苷酸的缺失或插入發(fā)生在內(nèi)含子區(qū);所有菌株編碼區(qū)核苷酸序列長度相同;在種內(nèi)水平氨基酸序列沒有差別,顯示出高度的保守性.利用最大簡約法(Maximum Parsimony)和鄰近結(jié)合法(Neighbor-joining)構(gòu)建系統(tǒng)發(fā)育樹,兩個系統(tǒng)發(fā)育樹的拓撲結(jié)構(gòu)相似,不同種在不同的分支上.Brn1基因適合突臍孢屬種級水平的分子系統(tǒng)學(xué)研究.

關(guān)鍵詞：系統(tǒng)發(fā)育樹;離蠕孢屬;序列分析

分類號：Q939.97 　文獻標識碼：A

文章編號：1672-6472(2004)04-0480-0486

基金項目：國家自然科學(xué)基金資助項目(項目編號:30170004)

作者簡介：孫廣宇,通訊作者 E-mail:sunguang@public.xa.sn.cn

作者單位：孫廣宇（西北農(nóng)林科技大學(xué)植保學(xué)院,陜西,楊凌,712100）　

　　　　　張雅梅（西北農(nóng)林科技大學(xué)植保學(xué)院,陜西,楊凌,712100）　

　　　　　張榮（西北農(nóng)林科技大學(xué)植保學(xué)院,陜西,楊凌,712100）　

參考文獻：

［1］Alcorn J L, 1988. A new species of Exserohilum. Trans Brit Mycol Soc. 90:146-148

［2］Berbee M L, Mona P, Hubbard S, 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia, 91 (6): 964～977

［3］Claudia A J, Victor M M, Lawrence E P et al., 1995. Comparison of nuclear ribosomal DNA sequence from Alternaria species pathogenic to crucifers. Mycol Res, 99 (5): 604～614

［4］Felsenstein J, 1993. PHYLIP: Phylogeny inference Package and Manual, 3.5c, Department of Genetics, University of Washington.Seattle.

［5］Nakada M,Tanaka C,Tsunewaki K et al., 1994. RFLP analysis for species separation in the genera Bipolaris and Curvularis.Mycoscience, 35:271 ～278

［6］Saitou N, Nei M, 1998. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol Biol Evol, 4:406～425

［7］Shimizu K, Tanaka C , Tsuda M, 1997. Cloning of Brnl, a reductase gene involved in melanin bio-synthesis in Cochliobolus heterostrophus.J Gen Appl Microbiol, 43:145～150

［8］Shimizu K, Tanaka C, Peng Y L et al., 1998. Phylogeny of Bipolaris inferred from nucleotide sequence of Brnl,a reductase gene involve in melanin biosynthesis.JGen Appl Microbiol, 44:251～258

［9］Sivanesan A, 1987. Graminicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their teleomorphs. Mycol Pap,158:203～261

［10］Sun G Y, Zhang R, Zhu M Q et a1.,1977. A new species of Exserohilum from China. Mycological Research, 101 (7): 776～778

［11］Sun G Y, Oide S, Tanaka E, et al., 2003. Species separation in Curvularia "geniculata" group inferred from Brnl gene sequences.Mycoscience, 44:239～244

［12］Sussman A S, 1968. Longevity and survivability of fungi. In: The Fungi, Vol 3, The fungal population, ed. by G. C. Ainsworth, A.S. Sussman, New York: Academic Press, 447～486

［13］Thompson J D, Gibson T J , Higgins D G, 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24:4876～4882

收稿日期：2004年3月8日

修稿日期：2004年5月20日

出版日期：2004年11月22日

菌物學(xué)報

MYCOSYSTEMA

2004 Vol.23 No.4 P.480-486