平菇子實(shí)體分化發(fā)育相關(guān)基因片段的克隆

Cloning of Differentiation and Development Related Gene Fragm ents in Pleurotus ostreatus

馬愛(ài)民　關(guān)海山　

摘　要：采用RAP-PCR差異顯示技術(shù)對(duì)平菇菌絲體及原基、菇蕾和成熟子實(shí)體進(jìn)行研究,獲得了在原基階段差異表達(dá)的3條cDNA片段POD1、POD2與POD3,經(jīng)過(guò)回收、重?cái)U(kuò)增、克隆與鑒定后進(jìn)行測(cè)序分析,初步的結(jié)果表明3條差異片段的長(zhǎng)度分別為745bp、482bp與477bp.同時(shí),國(guó)際聯(lián)網(wǎng)檢索查明片段POD3為HSP70基因片段.

關(guān)鍵詞：平菇; 差異顯示; RAP?PCR; 分化發(fā)育相關(guān)基因; HSP70

分類(lèi)號(hào)：Q344+.3; S646.1+4　文獻(xiàn)標(biāo)識(shí)碼：A

基金項(xiàng)目：國(guó)家自然科學(xué)基金資助項(xiàng)目(39770530)

作者簡(jiǎn)介：馬愛(ài)民,男,1965年生,碩士,副教授.

作者單位：馬愛(ài)民（華中農(nóng)業(yè)大學(xué)植物保護(hù)系武漢 430070）　

　　　　　關(guān)海山（香港中文大學(xué)生物系香港）　

參考文獻(xiàn)：

［1］Asgeirsdottir S A, De Veries O M H, Wessels J G H. Identification of th ree di fferentially expressed hydrophobins in Pleurotus ostreatus(oyster mushroom ). Microbiology, 1998,144:2961～2969

［2］Peng L, Aerthur B P. Differential display of eukaryotic messenger RNA by me ans of the polymerase chain reaction. Science, 1992,257:967～971

［3］Mcclelland M, Mathieu-Daude F, Welsh J. RNA fingerprinting and differential display using arbitrarily primed PCR. Trends Genet, 1995,11:242～246

［4］Plesofsk-Vig N, Brambl R. Topical review: the heat shock response of fungi. Experimental Mycology,1985,9:187～194

［5］Bonato M C M, Silva A M, Gomes S L, et al. Differential expression of heat- sh ock proteins and spontaneous synthesis of HSP70 during the life cycle of Blas tocladiella emersonii. Eur J Biochem, 1987, 163:211～220

［6］Fracella F, Schole C, Kallies A, et al. Differential HSC70 expression durin g asexual development of Neurospora crassa. Microbiolgy, 1997,143:3615～3624

華中農(nóng)業(yè)大學(xué)學(xué)報(bào)

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2001 Vol.20 No.4 P.299-302