草菇耐低溫菌株的誘變選育與鑒定

SELECTION FOR COLD-RESISTENT STRAIN BY COMPLEX MUTAGENESIS OF VOLVARIELLA VOLVACEA AND IDENTIFICATION OF THE STRAIN

韓業(yè)君　曹暉　陳明杰　潘迎捷　

摘　要：在草菇V23原生質(zhì)體形成和再生的最佳條件下制備原生質(zhì)體.分別用紫外線(UV),60Co-γ射線,硫酸二乙酯(DES)對原生質(zhì)體進行復(fù)合誘變.初篩、復(fù)篩獲得耐常規(guī)低溫(4℃)10d的菌株VH.栽培實驗證明:VH在26℃下的生物學(xué)效率明顯優(yōu)于V23(CK),且差異極顯著.同工酶分析表明:VH過氧化物同工酶,酯酶同工酶譜帶數(shù)量及酶活性均發(fā)生變化;RAPD實驗顯示:VH相對于V23的變異系數(shù)為0.213.VH菇體正常,味道鮮美,食用后無毒副作用.氨基酸組成分析表明:除總氨基酸和谷氨酸含量VH高于V23外,其它氨基酸差異不明顯.VH遺傳性狀穩(wěn)定,是具有潛在應(yīng)用價值的優(yōu)良菌株.

關(guān)鍵詞：原生質(zhì)體,同工酶分析,氨基酸

分類號：Q939.96　文獻標識碼：A

文章編號：1672-6472(2004)03-0417-0422

基金項目：上海市農(nóng)委科技興農(nóng)重點項目資助(農(nóng)科攻字(98)第01-14號)

作者簡介：陳明杰,通訊作者:mjchen@mail.china.com

作者單位：韓業(yè)君（南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院微生物系,南京,210095;農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點實驗室;上海市農(nóng)業(yè)科學(xué)院食用菌研究所,上海,201106）　

曹暉（農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點實驗室;上海市農(nóng)業(yè)科學(xué)院食用菌研究所,上海,201106）　

陳明杰（農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點實驗室;上海市農(nóng)業(yè)科學(xué)院食用菌研究所,上海,201106）　

潘迎捷（農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點實驗室;上海市農(nóng)業(yè)科學(xué)院食用菌研究所,上海,201106）　

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2004 Vol.23 No.3 P.417-422