蟲草多型現(xiàn)象的5.8S rDNA 和ITS2 序列測定研究

康冀川　梁宗琦　劉愛英　理查Y.C.孔　

摘　要：新定名的甘肅蟲草Cordycepsgansuensi與冬蟲夏草Cordycepssinensi形態(tài)相似。蛹蟲草Cordycepsmilitaris具有擬青霉型（Paecilomyces-type）和輪枝孢型（Verticillium-type）兩類無性型。此研究對5.8SrRNA基因及ITS2間區(qū)DNA序列的分析結果表明，甘肅蟲草和冬蟲夏草的DNA序列相同，屬于同一種；蛹蟲草的兩類無性型具有分子水平上的遺傳趨異性。

關鍵詞：冬蟲夏草，甘肅蟲草，蛹蟲草，分子研究，多型現(xiàn)象

分類號：Q939.96　文獻標識碼：A

文章編號：1007-3515（2000）04-0492-0497

MOLECULAR EVIDENCE OF POLYMORPHISM IN CORDYCEPS BASED ON 5.8S rDNA AND ITS2 SEQUENCES

KANG Ji-Chuan（Department of Plant Pathology, University of Stellenbosch, P.Bag X1, Matieland 7602, South Africa）　

LIANG Zong-Qi（Laboratory of Fungus Resources, Guizhou University, Guiyang 550025）　

LIU Ai-Ying（Laboratory of Fungus Resources, Guizhou University, Guiyang 550025）　

LIU Ai-Ying（Department of Biology and Chemistry, The City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong）　

Abstract：Cordyceps gansuensis, C. sinensis and C. militaris are reexamined in this study.Cordyceps gansuensis morphologically resembles C.sinensis, while C.militaris has two different types of anamorphs, the Paecilomyces-type and the Verticillium-type. Analysis of DNA sequences of the 5.8S rRNA gene and partial internal transcribed spacer (ITS2) region of the fungi indicate that C.gansuensis and C.sinensis are conspecific and that the two types of anamorphs of C.militaris are diverse at molecular level.

Keywords：Cordyceps sinensis, C.gansuensis, C.militaris, Molecular studies, Polymorphism.

基金項目：國家自然科學基金資助

作者單位：康冀川（Stellenbosch 大學植物病理系，南非 Matieland 7602）　

　　　　　梁宗琦（貴州大學真菌資源研究室，貴陽 550025）　

　　　　　劉愛英（貴州大學真菌資源研究室，貴陽 550025）　

　　　　　理查Y.C.孔（香港城市大學生物化學系，香港）　

參考文獻：

［1］Camichael JW, 1981. Biology of conidial fungi. vol 1,Academic press inc.1~514

［2］Devereux J, Haeberli P, Smithies O, 1984. Comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387～395

［3］Felsenstein J, 1993. PHYLIP: phylogeny inference package (version 3.5c). Distributed by the author, Department of Genetics, University of Washington, Seattle, USA

［4］Kobayasi Y, Shimizu D, 1993. Iconography of vegetable wasps nad plant worms. Hoikusha Pub Co Ltd Japan.1~625

［5］Lee SB,Taylor JW, 1990. Isolation of DNA from fungal mycelia and single spores. In PCR Protocols: A Guide to Methods and Applications, ed MA Innis, DH Gelfand, JS Sninsky, TJ White. New York: Academic press. 1～282

［6］Liang ZQ,1990. Anamorph of Cordyceps militaris and artificial culture of its fruitibody. Southwest China Journal of Agricultural Sciences 3, 1～6

［7］Liang ZQ, Liu AY, Liu JL, 1991. A new species of the genus Cordyceps and its Metarhizium anamorph. Acta Mycologica Sinica （真菌學報）10: 172～180

［8］Liang ZQ, Liu AY, Liu JL, 1995. The development of ascospore in Cordyceps sinensis (Berk.) Sacc. Acta

［9］Mycologica Sinica （真菌學報）14: 148～152

［10］Liang ZQ, Liu AY, 1996. The genus Cordyceps and its allies from Kuankuoshui Preserve in Guiahou I. Acta Mycologica Sinica （真菌學報）15: 264～271

［11］Liang ZQ , Liu AY, 1997. The genus Cordyceps and its allies from Kuankuoshui Preserve in Guiahou II. Mycosystema （菌物系統(tǒng)）16: 61～67

［12］Liu AY, Liang ZQ, 1997. Cordyceps spp. and some other entomopathogenic fungi from the Eemei Mountain Preserve in China I. Mycosystema （菌物系統(tǒng)）16: 13～143

［13］Liu ZY, Liang ZQ, 1996. A new species of Paecilomyces isolated from Cordyceps odonatae. Mycosystema （菌物系統(tǒng)）8: 83～87

［14］Liu ZY,LiangZQ, Liu AY,1997. A new fungus of the genus Cordyceps growing on white grub. Mycosystema （菌物系統(tǒng)）16: 4～8

［15］Saitou N, Nei M, 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406～425

［16］Thompson JD, Higgins DG,Gibson TJ, 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalites and weight matrix choice. Nucleic Acids Research 22: 4673～4680

［17］White TJ, Bruns TD, Lee S,Taylor JW, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols:A Guide to Methods and Applications, ed. MA Innis, DH Gelfand, JS Sninsky, TJ White, New York: Academic. 1～482

［18］Zhang K, Wang C, Yan M, 1989. A new species of Cordyceps from Gansu, China. Transactions of Mycological Society of Japan 30: 295～299